aspsirna0063 details

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ASPsiRNA information siRNA Id:aspsirna0063
siRNA Name: si(T11/C12)
Mismatch information Wild siRNA (As strand 3'->5'):GACUUCACUUCUACCUACGU
ASP-siRNA (As strand 3'->5'):GACUUCACUUAGACCUACGU
Mismatch position in siRNA: A10C,G9U
Gene Information Gene Name Amyloid beta (A4) precursor protein(APP)
Target Sequence (5'->3'):AATATCAAGACGGAGGAGATCTCTGAAGTGAAGATGGATGCAGAAT
Wild allele (5'->3'):CUGAAGUGAAGAUGGAUGCA
Mutant allele (5'->3'):CUGAAGUGAAUCUGGAUGCA
Position of siRNA on target gene: 2200-2219
Respective Gene/Protein Resources GenBank Accession:NM_000484.3
Cytogenic location:21q21.3
Chromosomal coordinates:21:27,252,860-27,543,445
UniProt ID:P56817
HUGO ID:620
Reference SNp(RefSNP): rs28186516
Disease/Mutation information Target Mutation: c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
Matation type/variant: Indel/missense variant
Mutation at gene level: NG_007376.1:g.278194_278195delGAinsTC
Pathogenic status of mutation: Pathogenic
Disease:Alzheimers disease
Clinical Resources ClinVar ID:18093
KEGG disease ID:H00056
OMIM ID: 104300
COSMIC: APP
DECIPHER: APP
GeneTests: APP
ASP siRNA details Mutant allele (5'->3'): CUGAAGUGAAUCUGGAUGCA
ASP-siRNA (As strand 3'->5'):GACUUCACUUAGACCUACGU
Percentage efficacy of ASP-siRNA for mutant allele:44
Wild allele (5'->3'): CUGAAGUGAAGAUGGAUGCA
ASP-siRNA (As strand 3'->5'):GACUUCACUUAGACCUACGU
Percentage efficacy of ASP-siRNA for wild allele:79
Relative difference:-35
Wild siRNA details Wild allele (5'->3'): CUGAAGUGAAGAUGGAUGCA
Wild siRNA (As strand 3'->5'):GACUUCACUUCUACCUACGU
Percentage efficacy of wild siRNA for wild allele:93
Wild allele (5'->3'): CUGAAGUGAAGAUGGAUGCA
ASP-siRNA (As strand 3'->5'):GACUUCACUUAGACCUACGU
Percentage efficacy of Wild sirna for mutant allele:65
Relative difference :28
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 24 hours
Concentration used:40nM
Reference:19771217
Delivery method:Transfection
Article title:Assessment of allele-specific gene silencing by RNA interference with mutant and wild-type reporter alleles.
Authors:Ohnishi Y, Tokunaga K, Kaneko K, Hohjoh H.
Journal Reference:J RNAi Gene Silencing. 2006 Feb 28;2(1):154-60.