aspsirna0097 details

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ASPsiRNA information siRNA Id:aspsirna0097
siRNA Name: siPrnp102(T9)-7C
Mismatch information Wild siRNA (As strand 3'->5'):CUUGUUCGGCUCAUUCGGU
ASP-siRNA (As strand 3'->5'):CUUGUUGGACUCAUUCGGU
Mismatch position in siRNA: G13C,A11G
Gene Information Gene Name Human prion protein (PRNP)
Target Sequence (5'->3'):AGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACA
Wild allele (5'->3'):GAACAAGCCGAGUAAGCCA
Mutant allele (5'->3'):GAACAACCUGAGUAAGCCA
Position of siRNA on target gene: 669-687
Respective Gene/Protein Resources GenBank Accession:NM_000311.3
Cytogenic location:20p13
Chromosomal coordinates:20:4,666,796-4,682,234
UniProt ID:P04156
HUGO ID:9449
Reference SNp(RefSNP): rs74315401
Disease/Mutation information Target Mutation: c.305C>T,p.Pro102Leu
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_009087.1:g.18375C>T
Pathogenic status of mutation: Pathogenic
Disease:Prion disease/Gerstmann-Straussler disease
Clinical Resources ClinVar ID:13395
KEGG disease ID:H00061
OMIM ID: 176640
COSMIC: PRNP
DECIPHER: PRNP
GeneTests: PRNP
ASP siRNA details Mutant allele (5'->3'): GAACAACCUGAGUAAGCCA
ASP-siRNA (As strand 3'->5'):CUUGUUGGACUCAUUCGGU
Percentage efficacy of ASP-siRNA for mutant allele:86
Wild allele (5'->3'): GAACAAGCCGAGUAAGCCA
ASP-siRNA (As strand 3'->5'):CUUGUUGGACUCAUUCGGU
Percentage efficacy of ASP-siRNA for wild allele:98
Relative difference:-12
Wild siRNA details Wild allele (5'->3'): GAACAAGCCGAGUAAGCCA
Wild siRNA (As strand 3'->5'):CUUGUUCGGCUCAUUCGGU
Percentage efficacy of wild siRNA for wild allele:90
Wild allele (5'->3'): GAACAAGCCGAGUAAGCCA
ASP-siRNA (As strand 3'->5'):CUUGUUGGACUCAUUCGGU
Percentage efficacy of Wild sirna for mutant allele:20
Relative difference :70
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Western blot
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 24 hours
Concentration used:40nM
Reference:18493311
Delivery method:shRNA expression vector delivery
Article title:Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi.
Authors:Ohnishi Y, Tamura Y, Yoshida M, Tokunaga K, Hohjoh H. PLoS One.
Journal Reference:2008 May 21;3(5):e2248. doi: 10.1371/journal.pone.0002248.