| | |
ASPsiRNA information |
siRNA Id: | aspsirna0126
|
siRNA Name: | siPrnp178(A9)-6G
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | AACACGUGCUGACGCAGUU
|
ASP-siRNA (As strand 3'->5'): | AACACCUGUUGACGCAGUU
|
Mismatch position in siRNA: | C14G,U11C |
Gene Information |
Gene Name | Human prion protein (PRNP) |
Target Sequence (5'->3'): | AGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACA
|
Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
|
Mutant allele (5'->3'): | UUGUGGACAACUGCGUCAA
|
Position of siRNA on target gene: | 896-914
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_000311.3 |
Cytogenic location: | 20p13 |
Chromosomal coordinates: | 20:4,666,796-4,682,234 |
UniProt ID: | P04156 |
HUGO ID: | 9449 |
Reference SNp(RefSNP): | rs74315403 |
Disease/Mutation information |
Target Mutation: | c.532G>A,p.Asp178Asn
|
Matation type/variant: | Single nucleotide variant/Missense variant
|
Mutation at gene level: | NG_009087.1:g.18602G>A
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Prion disease/Creutzfeldt-Jakob disease |
Clinical Resources |
ClinVar ID: | 39359 |
KEGG disease ID: | H00061 |
OMIM ID: | 176640 |
COSMIC: | PRNP |
DECIPHER: | PRNP |
GeneTests: | PRNP |
ASP siRNA details |
Mutant allele (5'->3'): | UUGUGGACAACUGCGUCAA
|
ASP-siRNA (As strand 3'->5'): | AACACCUGUUGACGCAGUU
|
Percentage efficacy of ASP-siRNA for mutant allele: | 52
|
Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
|
ASP-siRNA (As strand 3'->5'): | AACACCUGUUGACGCAGUU
|
Percentage efficacy of ASP-siRNA for wild allele: | 88
|
Relative difference: | -36
|
Wild siRNA details |
Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
|
Wild siRNA (As strand 3'->5'): | AACACGUGCUGACGCAGUU
|
Percentage efficacy of wild siRNA for wild allele: | 90
|
Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
|
ASP-siRNA (As strand 3'->5'): | AACACCUGUUGACGCAGUU
|
Percentage efficacy of Wild sirna for mutant allele: | 20
|
Relative difference : | 70
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | HEK293 |
Experimental technique used: | Western blot |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Invitrogen |
Post-transfection duration: | 24 hours |
Concentration used: | 40nM |
Reference: | 18493311 |
Delivery method: | shRNA expression vector delivery |
'Article title: | Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi. |
'Authors: | Ohnishi Y, Tamura Y, Yoshida M, Tokunaga K, Hohjoh H. PLoS One. |
'Journal Reference: | 2008 May 21;3(5):e2248. doi: 10.1371/journal.pone.0002248. |
'