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| ASPsiRNA information |
siRNA Id: | aspsirna0134
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siRNA Name: | siPrnp102(T9)-5U
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | CUUGUUCGGCUCAUUCGGU
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ASP-siRNA (As strand 3'->5'): | CUUGAUCGACUCAUUCGGU
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Mismatch position in siRNA: | A15U,A11G |
| Gene Information |
Gene Name | Human prion protein (PRNP) |
|
Target Sequence (5'->3'): | AGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACA
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Wild allele (5'->3'): | GAACAAGCCGAGUAAGCCA
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Mutant allele (5'->3'): | GAACUAGCUGAGUAAGCCA
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Position of siRNA on target gene: | 669-687
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| Respective Gene/Protein Resources |
GenBank Accession: | NM_000311.3 |
| Cytogenic location: | 20p13 |
| Chromosomal coordinates: | 20:4,666,796-4,682,234 |
| UniProt ID: | P04156 |
| HUGO ID: | 9449 |
|
Reference SNp(RefSNP): | rs74315401 |
| Disease/Mutation information |
Target Mutation: | c.305C>T,p.Pro102Leu
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_009087.1:g.18375C>T
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Pathogenic status of mutation: | Pathogenic
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Disease: | Prion disease/Gerstmann-Straussler disease |
| Clinical Resources |
ClinVar ID: | 13395 |
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KEGG disease ID: | H00061 |
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OMIM ID: | 176640 |
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COSMIC: | PRNP |
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DECIPHER: | PRNP |
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GeneTests: | PRNP |
| ASP siRNA details |
Mutant allele (5'->3'): | GAACUAGCUGAGUAAGCCA
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ASP-siRNA (As strand 3'->5'): | CUUGAUCGACUCAUUCGGU
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 98
|
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Wild allele (5'->3'): | GAACAAGCCGAGUAAGCCA
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ASP-siRNA (As strand 3'->5'): | CUUGAUCGACUCAUUCGGU
|
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Percentage efficacy of ASP-siRNA for wild allele: | 90
|
|
Relative difference: | 8
|
| Wild siRNA details |
Wild allele (5'->3'): | GAACAAGCCGAGUAAGCCA
|
|
Wild siRNA (As strand 3'->5'): | CUUGUUCGGCUCAUUCGGU
|
|
Percentage efficacy of wild siRNA for wild allele: | 90
|
|
Wild allele (5'->3'): | GAACAAGCCGAGUAAGCCA
|
|
ASP-siRNA (As strand 3'->5'): | CUUGAUCGACUCAUUCGGU
|
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Percentage efficacy of Wild sirna for mutant allele: | 20
|
|
Relative difference : | 70
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HeLa |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| Concentration used: | 40nM |
| Reference: | 18493311 |
| Delivery method: | shRNA expression vector delivery |
'| Article title: | Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi. |
'| Authors: | Ohnishi Y, Tamura Y, Yoshida M, Tokunaga K, Hohjoh H. PLoS One. |
'| Journal Reference: | 2008 May 21;3(5):e2248. doi: 10.1371/journal.pone.0002248. |
'
