aspsirna0166 details

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ASPsiRNA information siRNA Id:aspsirna0166
siRNA Name: siPrnp178(A9)-15C
Mismatch information Wild siRNA (As strand 3'->5'):AACACGUGCUGACGCAGUU
ASP-siRNA (As strand 3'->5'):AACACGUGUUGACGGAGUU
Mismatch position in siRNA: G5C,U11C
Gene Information Gene Name Human prion protein (PRNP)
Target Sequence (5'->3'):AGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACA
Wild allele (5'->3'):UUGUGCACGACUGCGUCAA
Mutant allele (5'->3'):UUGUGCACAACUGCCUCAA
Position of siRNA on target gene: 896-914
Respective Gene/Protein Resources GenBank Accession:NM_000311.3
Cytogenic location:20p13
Chromosomal coordinates:20:4,666,796-4,682,234
UniProt ID:P04156
HUGO ID:9449
Reference SNp(RefSNP): rs74315403
Disease/Mutation information Target Mutation: c.532G>A,p.Asp178Asn
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_009087.1:g.18602G>A
Pathogenic status of mutation: Pathogenic
Disease:Prion disease/Creutzfeldt-Jakob disease
Clinical Resources ClinVar ID:39359
KEGG disease ID:H00061
OMIM ID: 176640
COSMIC: PRNP
DECIPHER: PRNP
GeneTests: PRNP
ASP siRNA details Mutant allele (5'->3'): UUGUGCACAACUGCCUCAA
ASP-siRNA (As strand 3'->5'):AACACGUGUUGACGGAGUU
Percentage efficacy of ASP-siRNA for mutant allele:60
Wild allele (5'->3'): UUGUGCACGACUGCGUCAA
ASP-siRNA (As strand 3'->5'):AACACGUGUUGACGGAGUU
Percentage efficacy of ASP-siRNA for wild allele:88
Relative difference:-28
Wild siRNA details Wild allele (5'->3'): UUGUGCACGACUGCGUCAA
Wild siRNA (As strand 3'->5'):AACACGUGCUGACGCAGUU
Percentage efficacy of wild siRNA for wild allele:90
Wild allele (5'->3'): UUGUGCACGACUGCGUCAA
ASP-siRNA (As strand 3'->5'):AACACGUGUUGACGGAGUU
Percentage efficacy of Wild sirna for mutant allele:20
Relative difference :70
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Western blot
Transfection method: Lipofectamine 2000
siRNA expression method: Invitrogen
Post-transfection duration: 24 hours
Concentration used:40nM
Reference:18493311
Delivery method:shRNA expression vector delivery
Article title:Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi.
Authors:Ohnishi Y, Tamura Y, Yoshida M, Tokunaga K, Hohjoh H. PLoS One.
Journal Reference:2008 May 21;3(5):e2248. doi: 10.1371/journal.pone.0002248.