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| ASPsiRNA information |
siRNA Id: | aspsirna0166
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siRNA Name: | siPrnp178(A9)-15C
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | AACACGUGCUGACGCAGUU
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ASP-siRNA (As strand 3'->5'): | AACACGUGUUGACGGAGUU
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Mismatch position in siRNA: | G5C,U11C |
| Gene Information |
Gene Name | Human prion protein (PRNP) |
|
Target Sequence (5'->3'): | AGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACA
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Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
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Mutant allele (5'->3'): | UUGUGCACAACUGCCUCAA
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Position of siRNA on target gene: | 896-914
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| Respective Gene/Protein Resources |
GenBank Accession: | NM_000311.3 |
| Cytogenic location: | 20p13 |
| Chromosomal coordinates: | 20:4,666,796-4,682,234 |
| UniProt ID: | P04156 |
| HUGO ID: | 9449 |
|
Reference SNp(RefSNP): | rs74315403 |
| Disease/Mutation information |
Target Mutation: | c.532G>A,p.Asp178Asn
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_009087.1:g.18602G>A
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Pathogenic status of mutation: | Pathogenic
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Disease: | Prion disease/Creutzfeldt-Jakob disease |
| Clinical Resources |
ClinVar ID: | 39359 |
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KEGG disease ID: | H00061 |
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OMIM ID: | 176640 |
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COSMIC: | PRNP |
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DECIPHER: | PRNP |
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GeneTests: | PRNP |
| ASP siRNA details |
Mutant allele (5'->3'): | UUGUGCACAACUGCCUCAA
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ASP-siRNA (As strand 3'->5'): | AACACGUGUUGACGGAGUU
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 60
|
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Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
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ASP-siRNA (As strand 3'->5'): | AACACGUGUUGACGGAGUU
|
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Percentage efficacy of ASP-siRNA for wild allele: | 88
|
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Relative difference: | -28
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| Wild siRNA details |
Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
|
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Wild siRNA (As strand 3'->5'): | AACACGUGCUGACGCAGUU
|
|
Percentage efficacy of wild siRNA for wild allele: | 90
|
|
Wild allele (5'->3'): | UUGUGCACGACUGCGUCAA
|
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ASP-siRNA (As strand 3'->5'): | AACACGUGUUGACGGAGUU
|
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Percentage efficacy of Wild sirna for mutant allele: | 20
|
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Relative difference : | 70
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HeLa |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 24 hours |
| Concentration used: | 40nM |
| Reference: | 18493311 |
| Delivery method: | shRNA expression vector delivery |
'| Article title: | Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi. |
'| Authors: | Ohnishi Y, Tamura Y, Yoshida M, Tokunaga K, Hohjoh H. PLoS One. |
'| Journal Reference: | 2008 May 21;3(5):e2248. doi: 10.1371/journal.pone.0002248. |
'
