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| ASPsiRNA information |
siRNA Id: | aspsirna0170
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|
siRNA Name: | shAPPsw
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | ACUUCACUUCUACCUACGUC
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUAGACCUACGUC
|
|
Mismatch position in siRNA: | G10C,A11U |
| Gene Information |
Gene Name | Amyloid beta (A4) precursor protein(APP) |
|
Target Sequence (5'->3'): | TCTCTGAAGTGAAGATGGATGCAGAATTCCGAC
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|
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
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Mutant allele (5'->3'): | UGAAGUGAAUCUGGAUGCAG
|
|
Position of siRNA on target gene: | 2201-2220
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000484.3 |
| Cytogenic location: | 21q21.3 |
| Chromosomal coordinates: | 21:27,252,860-27,543,445 |
| UniProt ID: | P56817 |
| HUGO ID: | 620 |
|
Reference SNp(RefSNP): | rs28186516 |
| Disease/Mutation information |
Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
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Matation type/variant: | Indel/missense variant
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Mutation at gene level: | NG_007376.1:g.278194_278195delGAinsTC
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Pathogenic status of mutation: | Disease causing
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Disease: | Alzheimers disease |
| Clinical Resources |
ClinVar ID: | 18093 |
|
KEGG disease ID: | H00056 |
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OMIM ID: | 104300 |
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COSMIC: | APP |
|
DECIPHER: | APP |
|
GeneTests: | APP |
| ASP siRNA details |
Mutant allele (5'->3'): | UGAAGUGAAUCUGGAUGCAG
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUAGACCUACGUC
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 60
|
|
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUAGACCUACGUC
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 10
|
|
Relative difference: | 50
|
| Wild siRNA details |
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
|
|
Wild siRNA (As strand 3'->5'): | ACUUCACUUCUACCUACGUC
|
|
Percentage efficacy of wild siRNA for wild allele: | 68
|
|
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUAGACCUACGUC
|
|
Percentage efficacy of Wild sirna for mutant allele: | 2
|
|
Relative difference : | 66
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | Transgenic mouse model |
| Experimental technique used: | allele-specific qPCR |
| Transfection method: | Lipofectamine Plus(invitrogen) |
| siRNA expression method: | Invitrogen |
| Post-transfection duration: | 48 hours |
| Concentration used: | 0.01–6.25nM |
| Reference: | 19532137 |
| Delivery method: | Adenovirus associated shRNA delivery |
'| Article title: | Allele-specific RNAi mitigates phenotypic progression in a transgenic model of Alzheimer's disease. |
'| Authors: | Rodríguez-Lebrón E, Gouvion CM, Moore SA, Davidson BL, Paulson HL. |
'| Journal Reference: | Mol Ther. 2009 Sep;17(9):1563-73. doi: 10.1038/mt.2009.123. Epub 2009 Jun 16. |
'
