| | |
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| ASPsiRNA information |
siRNA Id: | aspsirna0190
|
|
siRNA Name: | siAPP plus G
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | NA
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUCUACCUACGUC
|
|
Mismatch position in siRNA: | NA |
| Gene Information |
Gene Name | Amyloid beta (A4) precursor protein(APP) |
|
Target Sequence (5'->3'): | GAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGA
|
|
Wild allele (5'->3'): | NA
|
|
Mutant allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
|
|
Position of siRNA on target gene: | 2201-2220
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000484.3 |
| Cytogenic location: | 21q21.3 |
| Chromosomal coordinates: | 21:27,252,860-27,543,445 |
| UniProt ID: | P05067 |
| HUGO ID: | 620 |
|
Reference SNp(RefSNP): | rs28186516 |
| Disease/Mutation information |
Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
|
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Matation type/variant: | Indel/missense variant
|
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Mutation at gene level: | NG_007376.1:g.278194_278195delGAinsTC
|
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Pathogenic status of mutation: | Pathogenic
|
|
Disease: | Alzheimers disease |
| Clinical Resources |
ClinVar ID: | 18093 |
|
KEGG disease ID: | H00056 |
|
OMIM ID: | 600274 |
|
COSMIC: | APP |
|
DECIPHER: | APP |
|
GeneTests: | APP |
| ASP siRNA details |
Mutant allele (5'->3'): | UGAAGUGAAGAUGGAUGCAG
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUCUACCUACGUC
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 83
|
|
Wild allele (5'->3'): | NA
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUCUACCUACGUC
|
|
Percentage efficacy of ASP-siRNA for wild allele: | Poor efficacy
|
|
Relative difference: | High discrimination
|
| Wild siRNA details |
Wild allele (5'->3'): | NA
|
|
Wild siRNA (As strand 3'->5'): | NA
|
|
Percentage efficacy of wild siRNA for wild allele: | Poor efficacy
|
|
Wild allele (5'->3'): | NA
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUCUACCUACGUC
|
|
Percentage efficacy of Wild sirna for mutant allele: | 83
|
|
Relative difference : | Moderate discrimination
|
| General information |
Mismatch incorporated in siRNA/Target: | nil |
| Cell-line used: | Cos-7 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Integrated DNA Technologies |
| Post-transfection duration: | 72 hours |
| Concentration used: | 10mg |
| Reference: | 14754988 |
| Delivery method: | shRNA expression plasmid delivery |
'| Article title: | Targeting Alzheimer's disease genes with RNA interference: an efficient strategy for silencing mutant alleles. |
'| Authors: | Miller VM, Gouvion CM, Davidson BL, Paulson HL. |
'| Journal Reference: | Nucleic Acids Res. 2004 Jan 30;32(2):661-8. Print 2004. |
'
