| | |
ASPsiRNA information |
siRNA Id: | aspsirna0191
|
siRNA Name: | siAPP
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | NA
|
ASP-siRNA (As strand 3'->5'): | UUCACUUCUACCUACGUCUUAAG
|
Mismatch position in siRNA: | NA |
Gene Information |
Gene Name | Amyloid beta (A4) precursor protein(APP) |
Target Sequence (5'->3'): | GAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGA
|
Wild allele (5'->3'): | NA
|
Mutant allele (5'->3'): | AAGUGAAGAUGGAUGCAGAAUUC
|
Position of siRNA on target gene: | 2203-2225
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_000484.3 |
Cytogenic location: | 21q21.3 |
Chromosomal coordinates: | 21:27,252,860-27,543,445 |
UniProt ID: | P10636 |
HUGO ID: | 6893 |
Reference SNp(RefSNP): | rs28186516 |
Disease/Mutation information |
Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
|
Matation type/variant: | Indel/missense variant
|
Mutation at gene level: | NG_007376.1:g.278194_278195delGAinsTC
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Alzheimers disease |
Clinical Resources |
ClinVar ID: | 18093 |
KEGG disease ID: | H00056 |
OMIM ID: | 600274 |
COSMIC: | APP |
DECIPHER: | APP |
GeneTests: | APP |
ASP siRNA details |
Mutant allele (5'->3'): | AAGUGAAGAUGGAUGCAGAAUUC
|
ASP-siRNA (As strand 3'->5'): | UUCACUUCUACCUACGUCUUAAG
|
Percentage efficacy of ASP-siRNA for mutant allele: | NA
|
Wild allele (5'->3'): | NA
|
ASP-siRNA (As strand 3'->5'): | UUCACUUCUACCUACGUCUUAAG
|
Percentage efficacy of ASP-siRNA for wild allele: | NA
|
Relative difference: | NA
|
Wild siRNA details |
Wild allele (5'->3'): | NA
|
Wild siRNA (As strand 3'->5'): | NA
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
Wild allele (5'->3'): | NA
|
ASP-siRNA (As strand 3'->5'): | UUCACUUCUACCUACGUCUUAAG
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
General information |
Mismatch incorporated in siRNA/Target: | nil |
Cell-line used: | Cos-7 |
Experimental technique used: | Western blot |
Transfection method: | Lipofectamine Plus |
siRNA expression method: | Integrated DNA Technologies |
Post-transfection duration: | 72 hours |
Concentration used: | 10mg |
Reference: | 14754988 |
Delivery method: | shRNA expression plasmid delivery |
'Article title: | Targeting Alzheimer's disease genes with RNA interference: an efficient strategy for silencing mutant alleles. |
'Authors: | Miller VM, Gouvion CM, Davidson BL, Paulson HL. |
'Journal Reference: | Nucleic Acids Res. 2004 Jan 30;32(2):661-8. Print 2004. |
'