aspsirna0193 details

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ASPsiRNA information siRNA Id:aspsirna0193
siRNA Name: siT9/C10
Mismatch information Wild siRNA (As strand 3'->5'):CUUCACUUCUACCUACGUCU
ASP-siRNA (As strand 3'->5'):CUUCACUUAGACCUACGUCU
Mismatch position in siRNA: A12C,G11U
Gene Information Gene Name Amyloid beta (A4) precursor protein(APP)
Target Sequence (5'->3'):GAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGA
Wild allele (5'->3'):GAAGUGAAGAUGGAUGCAGA
Mutant allele (5'->3'):GAAGUGAAUCUGGAUGCAGA
Position of siRNA on target gene: 2202-2221
Respective Gene/Protein Resources GenBank Accession:NM_000484.3
Cytogenic location:21q21.3
Chromosomal coordinates:21:27,252,860-27,543,445
UniProt ID:P05067
HUGO ID:620
Reference SNp(RefSNP): rs28186516
Disease/Mutation information Target Mutation: c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
Matation type/variant: Indel/missense variant
Mutation at gene level: NG_007376.1:g.278194_278195delGAinsTC
Pathogenic status of mutation: Pathogenic
Disease:Alzheimers disease
Clinical Resources ClinVar ID:18093
KEGG disease ID:H00056
OMIM ID: 104300
COSMIC: APP
DECIPHER: APP
GeneTests: APP
ASP siRNA details Mutant allele (5'->3'): GAAGUGAAUCUGGAUGCAGA
ASP-siRNA (As strand 3'->5'):CUUCACUUAGACCUACGUCU
Percentage efficacy of ASP-siRNA for mutant allele:65
Wild allele (5'->3'): GAAGUGAAGAUGGAUGCAGA
ASP-siRNA (As strand 3'->5'):CUUCACUUAGACCUACGUCU
Percentage efficacy of ASP-siRNA for wild allele:Poor efficacy
Relative difference:High discrimination
Wild siRNA details Wild allele (5'->3'): GAAGUGAAGAUGGAUGCAGA
Wild siRNA (As strand 3'->5'):CUUCACUUCUACCUACGUCU
Percentage efficacy of wild siRNA for wild allele:High efficacy
Wild allele (5'->3'): GAAGUGAAGAUGGAUGCAGA
ASP-siRNA (As strand 3'->5'):CUUCACUUAGACCUACGUCU
Percentage efficacy of Wild sirna for mutant allele:87
Relative difference :Poor discrimination
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Cos-7
Experimental technique used: Western blot
Transfection method: Lipofectamine Plus
siRNA expression method: Integrated DNA Technologies
Post-transfection duration: 72 hours
Concentration used:10mg
Reference:14754988
Delivery method:shRNA expression plasmid delivery
Article title:Targeting Alzheimer's disease genes with RNA interference: an efficient strategy for silencing mutant alleles.
Authors:Miller VM, Gouvion CM, Davidson BL, Paulson HL.
Journal Reference:Nucleic Acids Res. 2004 Jan 30;32(2):661-8. Print 2004.