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| ASPsiRNA information |
siRNA Id: | aspsirna0195
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siRNA Name: | siT11/C12
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | GACUUCACUUCUACCUACGU
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ASP-siRNA (As strand 3'->5'): | GACUUCACUUAGACCUACGU
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Mismatch position in siRNA: | A10C,G9U |
| Gene Information |
Gene Name | Amyloid beta (A4) precursor protein(APP) |
|
Target Sequence (5'->3'): | GAGATCTCTGAAGTGAAGATGGATGCAGAATTCCGACATGA
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Wild allele (5'->3'): | CUGAAGUGAAGAUGGAUGCA
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Mutant allele (5'->3'): | CUGAAGUGAAUCUGGAUGCA
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Position of siRNA on target gene: | 2200-2219
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| Respective Gene/Protein Resources |
GenBank Accession: | NM_000484.3 |
| Cytogenic location: | 21q21.3 |
| Chromosomal coordinates: | 21:27,252,860-27,543,445 |
| UniProt ID: | P05067 |
| HUGO ID: | 620 |
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Reference SNp(RefSNP): | rs28186516 |
| Disease/Mutation information |
Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
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Matation type/variant: | Indel/missense variant
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Mutation at gene level: | NG_007376.1:g.278194_278195delGAinsTC
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Pathogenic status of mutation: | Pathogenic
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Disease: | Alzheimers disease |
| Clinical Resources |
ClinVar ID: | 18093 |
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KEGG disease ID: | H00056 |
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OMIM ID: | 104300 |
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COSMIC: | APP |
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DECIPHER: | APP |
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GeneTests: | APP |
| ASP siRNA details |
Mutant allele (5'->3'): | CUGAAGUGAAUCUGGAUGCA
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ASP-siRNA (As strand 3'->5'): | GACUUCACUUAGACCUACGU
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Percentage efficacy of ASP-siRNA for mutant allele: | 45
|
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Wild allele (5'->3'): | CUGAAGUGAAGAUGGAUGCA
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ASP-siRNA (As strand 3'->5'): | GACUUCACUUAGACCUACGU
|
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Percentage efficacy of ASP-siRNA for wild allele: | Poor efficacy
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Relative difference: | Moderate discrimination
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| Wild siRNA details |
Wild allele (5'->3'): | CUGAAGUGAAGAUGGAUGCA
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|
Wild siRNA (As strand 3'->5'): | GACUUCACUUCUACCUACGU
|
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Percentage efficacy of wild siRNA for wild allele: | High efficacy
|
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Wild allele (5'->3'): | CUGAAGUGAAGAUGGAUGCA
|
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ASP-siRNA (As strand 3'->5'): | GACUUCACUUAGACCUACGU
|
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Percentage efficacy of Wild sirna for mutant allele: | 87
|
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Relative difference : | Poor discrimination
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | Cos-7 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Integrated DNA Technologies |
| Post-transfection duration: | 72 hours |
| Concentration used: | 10mg |
| Reference: | 14754988 |
| Delivery method: | shRNA expression plasmid delivery |
'| Article title: | Targeting Alzheimer's disease genes with RNA interference: an efficient strategy for silencing mutant alleles. |
'| Authors: | Miller VM, Gouvion CM, Davidson BL, Paulson HL. |
'| Journal Reference: | Nucleic Acids Res. 2004 Jan 30;32(2):661-8. Print 2004. |
'
