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| ASPsiRNA information |
siRNA Id: | aspsirna0197
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siRNA Name: | siA9
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CACCGGUCCACCUUCAUUUUAG
|
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ASP-siRNA (As strand 3'->5'): | CACCGGUCUACCUUCAUUUUAG
|
|
Mismatch position in siRNA: | U14C |
| Gene Information |
Gene Name | Microtubule-associated protein tau (MAPT) |
|
Target Sequence (5'->3'): | ACCAGGAGGTGGCCAGGTGGAAGTAAAATCTGA
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Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
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Mutant allele (5'->3'): | GUGGCCAGAUGGAAGUAAAAUC
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Position of siRNA on target gene: | 2274-2295
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_016835.4 |
| Cytogenic location: | 17q21.31 |
| Chromosomal coordinates: | 17:43,971,701-44,105,699 |
| UniProt ID: | P10636 |
| HUGO ID: | 6893 |
|
Reference SNp(RefSNP): | rs63750570 |
| Disease/Mutation information |
Target Mutation: | c.1960G>A,p.Val654Met
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_007398.1:g.129219G>A
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Pathogenic status of mutation: | Pathogenic
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Disease: | Frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17) |
| Clinical Resources |
ClinVar ID: | 14252 |
|
KEGG disease ID: | H00078 |
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OMIM ID: | 600274 |
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COSMIC: | MAPT |
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DECIPHER: | MAPT |
|
GeneTests: | MAPT |
| ASP siRNA details |
Mutant allele (5'->3'): | GUGGCCAGAUGGAAGUAAAAUC
|
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ASP-siRNA (As strand 3'->5'): | CACCGGUCUACCUUCAUUUUAG
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 65
|
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Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
|
|
ASP-siRNA (As strand 3'->5'): | CACCGGUCUACCUUCAUUUUAG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 60
|
|
Relative difference: | 5
|
| Wild siRNA details |
Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
|
|
Wild siRNA (As strand 3'->5'): | CACCGGUCCACCUUCAUUUUAG
|
|
Percentage efficacy of wild siRNA for wild allele: | High efficacy
|
|
Wild allele (5'->3'): | GUGGCCAGGUGGAAGUAAAAUC
|
|
ASP-siRNA (As strand 3'->5'): | CACCGGUCUACCUUCAUUUUAG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 87
|
|
Relative difference : | Poor discrimination
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | Cos-7 |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Integrated DNA Technologies |
| Post-transfection duration: | 72 hours |
| Concentration used: | 10mg |
| Reference: | 14754988 |
| Delivery method: | shRNA expression plasmid delivery |
'| Article title: | Targeting Alzheimer's disease genes with RNA interference: an efficient strategy for silencing mutant alleles. |
'| Authors: | Miller VM, Gouvion CM, Davidson BL, Paulson HL. |
'| Journal Reference: | Nucleic Acids Res. 2004 Jan 30;32(2):661-8. Print 2004. |
'
