| | |
|---|
| ASPsiRNA information |
siRNA Id: | aspsirna0226
|
|
siRNA Name: | Alpha-SYN-P1617
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CGUCGUCCUUUCUGUUUUC
|
|
ASP-siRNA (As strand 3'->5'): | CGCGGUCCUUUCUGUUUUC
|
|
Mismatch position in siRNA: | G16C,C17U |
| Gene Information |
Gene Name | Synuclein, alpha (SNCA) |
|
Target Sequence (5'->3'): | AACAGGGUGUGGCAGAAGCAGCAGGAAAGACAAAGAGG
|
|
Wild allele (5'->3'): | GCAGCAGGAAAGACAAAAG
|
|
Mutant allele (5'->3'): | GCGCCAGGAAAGACAAAAG
|
|
Position of siRNA on target gene: | NA
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000345.3 |
| Cytogenic location: | 4q22.1 |
| Chromosomal coordinates: | 4:90,645,249-90,759,446 |
| UniProt ID: | P37840 |
| HUGO ID: | 11138 |
|
Reference SNp(RefSNP): | rs10489387 |
| Disease/Mutation information |
Target Mutation: | c.88G>C,p.Ala30Pro
|
|
Matation type/variant: | Single nucleotide variant/Missense variant
|
|
Mutation at gene level: | NG_011851.1:g.7717G>C
|
|
Pathogenic status of mutation: | Pathogenic
|
|
Disease: | Parkinsons disease (PD) |
| Clinical Resources |
ClinVar ID: | 14008 |
|
KEGG disease ID: | H00057 |
|
OMIM ID: | 168600 |
|
COSMIC: | SNCA |
|
DECIPHER: | SNCA |
|
GeneTests: | SNCA |
| ASP siRNA details |
Mutant allele (5'->3'): | GCGCCAGGAAAGACAAAAG
|
|
ASP-siRNA (As strand 3'->5'): | CGCGGUCCUUUCUGUUUUC
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 80
|
|
Wild allele (5'->3'): | GCAGCAGGAAAGACAAAAG
|
|
ASP-siRNA (As strand 3'->5'): | CGCGGUCCUUUCUGUUUUC
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 70
|
|
Relative difference: | 10
|
| Wild siRNA details |
Wild allele (5'->3'): | GCAGCAGGAAAGACAAAAG
|
|
Wild siRNA (As strand 3'->5'): | CGUCGUCCUUUCUGUUUUC
|
|
Percentage efficacy of wild siRNA for wild allele: | High efficacy
|
|
Wild allele (5'->3'): | GCAGCAGGAAAGACAAAAG
|
|
ASP-siRNA (As strand 3'->5'): | CGCGGUCCUUUCUGUUUUC
|
|
Percentage efficacy of Wild sirna for mutant allele: | Moderate efficacy
|
|
Relative difference : | Poor discrimination
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Flourescence microscopy |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Sigma Genosys |
| Post-transfection duration: | 48 hours |
| Concentration used: | 100nM |
| Reference: | 22031823 |
| Delivery method: | shRNA expression vector delivery |
'| Article title: | Identification of allele-specific RNAi effectors targeting genetic forms of Parkinson's disease. |
'| Authors: | Sibley CR, Wood MJ. |
'| Journal Reference: | PLoS One. 2011;6(10):e26194. doi: 10.1371/journal.pone.0026194. Epub 2011 Oct 21. |
'
