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| ASPsiRNA information |
siRNA Id: | aspsirna0234
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siRNA Name: | Alpha-SYN-P1011
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | CGUCUUCGUCGUCCUUUCU
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ASP-siRNA (As strand 3'->5'): | CGUCUUCGCGGUCCUUUCU
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Mismatch position in siRNA: | G10C,C11U |
| Gene Information |
Gene Name | Synuclein, alpha (SNCA) |
|
Target Sequence (5'->3'): | AACAGGGUGUGGCAGAAGCAGCAGGAAAGACAAAGAGG
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Wild allele (5'->3'): | GCAGAAGCAGCAGGAAAGA
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Mutant allele (5'->3'): | GCAGAAGCGCCAGGAAAGA
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Position of siRNA on target gene: | 342-360
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000345.3 |
| Cytogenic location: | 4q22.1 |
| Chromosomal coordinates: | 4:90,645,249-90,759,446 |
| UniProt ID: | P37840 |
| HUGO ID: | 11138 |
|
Reference SNp(RefSNP): | rs10489387 |
| Disease/Mutation information |
Target Mutation: | c.88G>C,p.Ala30Pro
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_011851.1:g.7717G>C
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Pathogenic status of mutation: | Pathogenic
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Disease: | Parkinsons disease (PD) |
| Clinical Resources |
ClinVar ID: | 14008 |
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KEGG disease ID: | H00057 |
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OMIM ID: | 168600 |
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COSMIC: | SNCA |
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DECIPHER: | SNCA |
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GeneTests: | SNCA |
| ASP siRNA details |
Mutant allele (5'->3'): | GCAGAAGCGCCAGGAAAGA
|
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ASP-siRNA (As strand 3'->5'): | CGUCUUCGCGGUCCUUUCU
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 15
|
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Wild allele (5'->3'): | GCAGAAGCAGCAGGAAAGA
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ASP-siRNA (As strand 3'->5'): | CGUCUUCGCGGUCCUUUCU
|
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Percentage efficacy of ASP-siRNA for wild allele: | 40
|
|
Relative difference: | -25
|
| Wild siRNA details |
Wild allele (5'->3'): | GCAGAAGCAGCAGGAAAGA
|
|
Wild siRNA (As strand 3'->5'): | CGUCUUCGUCGUCCUUUCU
|
|
Percentage efficacy of wild siRNA for wild allele: | High efficacy
|
|
Wild allele (5'->3'): | GCAGAAGCAGCAGGAAAGA
|
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ASP-siRNA (As strand 3'->5'): | CGUCUUCGCGGUCCUUUCU
|
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Percentage efficacy of Wild sirna for mutant allele: | Moderate efficacy
|
|
Relative difference : | Poor discrimination
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Sigma Genosys |
| Post-transfection duration: | 48 hours |
| Concentration used: | 100nM |
| Reference: | 22031823 |
| Delivery method: | shRNA expression vector delivery |
'| Article title: | Identification of allele-specific RNAi effectors targeting genetic forms of Parkinson's disease. |
'| Authors: | Sibley CR, Wood MJ. |
'| Journal Reference: | PLoS One. 2011;6(10):e26194. doi: 10.1371/journal.pone.0026194. Epub 2011 Oct 21. |
'
