aspsirna0240 details

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ASPsiRNA information siRNA Id:aspsirna0240
siRNA Name: Alpha-SYN-P1617
Mismatch information Wild siRNA (As strand 3'->5'):CGUCGUCCUUUCUGUUUUC
ASP-siRNA (As strand 3'->5'):CGCGGUCCUUUCUGUUUUC
Mismatch position in siRNA: G16C,C17U
Gene Information Gene Name Synuclein, alpha (SNCA)
Target Sequence (5'->3'):AACAGGGUGUGGCAGAAGCAGCAGGAAAGACAAAGAGG
Wild allele (5'->3'):GCAGCAGGAAAGACAAAAG
Mutant allele (5'->3'):GCGCCAGGAAAGACAAAAG
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_000345.3
Cytogenic location:4q22.1
Chromosomal coordinates:4:90,645,249-90,759,446
UniProt ID:P37840
HUGO ID:18618
Reference SNp(RefSNP): rs10489387
Disease/Mutation information Target Mutation: c.88G>C,p.Ala30Pro
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_011851.1:g.7717G>C
Pathogenic status of mutation: Pathogenic
Disease:Parkinsons disease (PD)
Clinical Resources ClinVar ID:14008
KEGG disease ID:H00057
OMIM ID: 168600
COSMIC: SNCA
DECIPHER: SNCA
GeneTests: SNCA
ASP siRNA details Mutant allele (5'->3'): GCGCCAGGAAAGACAAAAG
ASP-siRNA (As strand 3'->5'):CGCGGUCCUUUCUGUUUUC
Percentage efficacy of ASP-siRNA for mutant allele:70
Wild allele (5'->3'): GCAGCAGGAAAGACAAAAG
ASP-siRNA (As strand 3'->5'):CGCGGUCCUUUCUGUUUUC
Percentage efficacy of ASP-siRNA for wild allele:60
Relative difference:10
Wild siRNA details Wild allele (5'->3'): GCAGCAGGAAAGACAAAAG
Wild siRNA (As strand 3'->5'):CGUCGUCCUUUCUGUUUUC
Percentage efficacy of wild siRNA for wild allele:High efficacy
Wild allele (5'->3'): GCAGCAGGAAAGACAAAAG
ASP-siRNA (As strand 3'->5'):CGCGGUCCUUUCUGUUUUC
Percentage efficacy of Wild sirna for mutant allele:Moderate efficacy
Relative difference :Poor discrimination
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Sigma Genosys
Post-transfection duration: 48 hours
Concentration used:100nM
Reference:22031823
Delivery method:shRNA expression vector delivery
Article title:Identification of allele-specific RNAi effectors targeting genetic forms of Parkinson's disease.
Authors:Sibley CR, Wood MJ.
Journal Reference:PLoS One. 2011;6(10):e26194. doi: 10.1371/journal.pone.0026194. Epub 2011 Oct 21.