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| ASPsiRNA information |
siRNA Id: | aspsirna0245
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siRNA Name: | LRRK2 P5
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | UCUAACGACUGAUGCCGUA
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ASP-siRNA (As strand 3'->5'): | UCUAACGACUGAUGUCGUA
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Mismatch position in siRNA: | U5C |
| Gene Information |
Gene Name | Leucine rich repeat kinase 2 (LRRK2) Gene |
|
Target Sequence (5'->3'): | UUGCAAAGAUUGCUGACUACGGCAUUGCUCAGUACUGC
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Wild allele (5'->3'): | AGAUUGCUGACUACGGCAU
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Mutant allele (5'->3'): | AGAUUGCUGACUACAGCAU
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Position of siRNA on target gene: | 6162-6180
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_198578.3 |
| Cytogenic location: | 12q12 |
| Chromosomal coordinates: | 12:40,618,812-40,763,086 |
| UniProt ID: | Q5S007 |
| HUGO ID: | 18618 |
|
Reference SNp(RefSNP): | rs34637584 |
| Disease/Mutation information |
Target Mutation: | c.6055G>A,p.Gly2019Ser
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_011709.1:g.120390G>A
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Pathogenic status of mutation: | Pathogenic
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Disease: | Parkinsons disease (PD) |
| Clinical Resources |
ClinVar ID: | 1940 |
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KEGG disease ID: | H00057 |
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OMIM ID: | 168600 |
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COSMIC: | LRRK2 |
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DECIPHER: | LRRK2 |
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GeneTests: | LRRK2 |
| ASP siRNA details |
Mutant allele (5'->3'): | AGAUUGCUGACUACAGCAU
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ASP-siRNA (As strand 3'->5'): | UCUAACGACUGAUGUCGUA
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 13
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Wild allele (5'->3'): | AGAUUGCUGACUACGGCAU
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ASP-siRNA (As strand 3'->5'): | UCUAACGACUGAUGUCGUA
|
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Percentage efficacy of ASP-siRNA for wild allele: | 88
|
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Relative difference: | -75
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| Wild siRNA details |
Wild allele (5'->3'): | AGAUUGCUGACUACGGCAU
|
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Wild siRNA (As strand 3'->5'): | UCUAACGACUGAUGCCGUA
|
|
Percentage efficacy of wild siRNA for wild allele: | High efficacy
|
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Wild allele (5'->3'): | AGAUUGCUGACUACGGCAU
|
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ASP-siRNA (As strand 3'->5'): | UCUAACGACUGAUGUCGUA
|
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Percentage efficacy of Wild sirna for mutant allele: | Moderate efficacy
|
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Relative difference : | Poor discrimination
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Sigma Genosys |
| Post-transfection duration: | 48 hours |
| Concentration used: | 50nM |
| Reference: | 22031823 |
| Delivery method: | shRNA expression vector delivery |
'| Article title: | Identification of allele-specific RNAi effectors targeting genetic forms of Parkinson's disease. |
'| Authors: | Sibley CR, Wood MJ. |
'| Journal Reference: | PLoS One. 2011;6(10):e26194. doi: 10.1371/journal.pone.0026194. Epub 2011 Oct 21. |
'
