| | |
ASPsiRNA information |
siRNA Id: | aspsirna0259
|
siRNA Name: | LRRK2 P14_10G
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | UGAUGCCGUAACGAGUCAU
|
ASP-siRNA (As strand 3'->5'): | UGAUGUCGUGACGAGUCAU
|
Mismatch position in siRNA: | U14C,G10A |
Gene Information |
Gene Name | Leucine rich repeat kinase 2 (LRRK2) Gene |
Target Sequence (5'->3'): | UUGCAAAGAUUGCUGACUACGGCAUUGCUCAGUACUGC
|
Wild allele (5'->3'): | ACUACGGCAUUGCUCAGUA
|
Mutant allele (5'->3'): | ACUACAGCACUGCUCAGUA
|
Position of siRNA on target gene: | 6171-6189
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_198578.3 |
Cytogenic location: | 12q12 |
Chromosomal coordinates: | 12:40,618,812-40,763,086 |
UniProt ID: | Q5S007 |
HUGO ID: | 18618 |
Reference SNp(RefSNP): | rs34637584 |
Disease/Mutation information |
Target Mutation: | c.6055G>A,p.Gly2019Ser
|
Matation type/variant: | Single nucleotide variant/Missense variant
|
Mutation at gene level: | NG_011709.1:g.120390G>A
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Parkinsons disease (PD) |
Clinical Resources |
ClinVar ID: | 1940 |
KEGG disease ID: | H00057 |
OMIM ID: | 168600 |
COSMIC: | LRRK2 |
DECIPHER: | LRRK2 |
GeneTests: | LRRK2 |
ASP siRNA details |
Mutant allele (5'->3'): | ACUACAGCACUGCUCAGUA
|
ASP-siRNA (As strand 3'->5'): | UGAUGUCGUGACGAGUCAU
|
Percentage efficacy of ASP-siRNA for mutant allele: | 76
|
Wild allele (5'->3'): | ACUACGGCAUUGCUCAGUA
|
ASP-siRNA (As strand 3'->5'): | UGAUGUCGUGACGAGUCAU
|
Percentage efficacy of ASP-siRNA for wild allele: | 45
|
Relative difference: | 31
|
Wild siRNA details |
Wild allele (5'->3'): | ACUACGGCAUUGCUCAGUA
|
Wild siRNA (As strand 3'->5'): | UGAUGCCGUAACGAGUCAU
|
Percentage efficacy of wild siRNA for wild allele: | High efficacy
|
Wild allele (5'->3'): | ACUACGGCAUUGCUCAGUA
|
ASP-siRNA (As strand 3'->5'): | UGAUGUCGUGACGAGUCAU
|
Percentage efficacy of Wild sirna for mutant allele: | Moderate efficacy
|
Relative difference : | Poor discrimination
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | HEK293 |
Experimental technique used: | Flourescence microscopy |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Sigma Genosys |
Post-transfection duration: | 48 hours |
Concentration used: | 50nM |
Reference: | 22031823 |
Delivery method: | shRNA expression vector delivery |
'Article title: | Identification of allele-specific RNAi effectors targeting genetic forms of Parkinson's disease. |
'Authors: | Sibley CR, Wood MJ. |
'Journal Reference: | PLoS One. 2011;6(10):e26194. doi: 10.1371/journal.pone.0026194. Epub 2011 Oct 21. |
'