aspsirna0262 details

''''
ASPsiRNA information siRNA Id:aspsirna0262
siRNA Name: LRRK2 P14_15C
Mismatch information Wild siRNA (As strand 3'->5'):UGAUGCCGUAACGAGUCAU
ASP-siRNA (As strand 3'->5'):UGAUCUCGUAACGAGUCAU
Mismatch position in siRNA: U14C, C15G
Gene Information Gene Name Leucine rich repeat kinase 2 (LRRK2) Gene
Target Sequence (5'->3'):UUGCAAAGAUUGCUGACUACGGCAUUGCUCAGUACUGC
Wild allele (5'->3'):ACUACGGCAUUGCUCAGUA
Mutant allele (5'->3'):ACUAGAGCAUUGCUCAGUA
Position of siRNA on target gene: 6171-6189
Respective Gene/Protein Resources GenBank Accession:NM_198578.3
Cytogenic location:12q12
Chromosomal coordinates:12:40,618,812-40,763,086
UniProt ID:Q5S007
HUGO ID:18618
Reference SNp(RefSNP): rs34637584
Disease/Mutation information Target Mutation: c.6055G>A,p.Gly2019Ser
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_011709.1:g.120390G>A
Pathogenic status of mutation: Pathogenic
Disease:Parkinsons disease (PD)
Clinical Resources ClinVar ID:1940
KEGG disease ID:H00057
OMIM ID: 168600
COSMIC: LRRK2
DECIPHER: LRRK2
GeneTests: LRRK2
ASP siRNA details Mutant allele (5'->3'): ACUAGAGCAUUGCUCAGUA
ASP-siRNA (As strand 3'->5'):UGAUCUCGUAACGAGUCAU
Percentage efficacy of ASP-siRNA for mutant allele:67
Wild allele (5'->3'): ACUACGGCAUUGCUCAGUA
ASP-siRNA (As strand 3'->5'):UGAUCUCGUAACGAGUCAU
Percentage efficacy of ASP-siRNA for wild allele:49
Relative difference:18
Wild siRNA details Wild allele (5'->3'): ACUACGGCAUUGCUCAGUA
Wild siRNA (As strand 3'->5'):UGAUGCCGUAACGAGUCAU
Percentage efficacy of wild siRNA for wild allele:High efficacy
Wild allele (5'->3'): ACUACGGCAUUGCUCAGUA
ASP-siRNA (As strand 3'->5'):UGAUCUCGUAACGAGUCAU
Percentage efficacy of Wild sirna for mutant allele:Moderate efficacy
Relative difference :Poor discrimination
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Flourescence microscopy
Transfection method: Lipofectamine 2000
siRNA expression method: Sigma Genosys
Post-transfection duration: 48 hours
Concentration used:50nM
Reference:22031823
Delivery method:shRNA expression vector delivery
Article title:Identification of allele-specific RNAi effectors targeting genetic forms of Parkinson's disease.
Authors:Sibley CR, Wood MJ.
Journal Reference:PLoS One. 2011;6(10):e26194. doi: 10.1371/journal.pone.0026194. Epub 2011 Oct 21.