aspsirna0347 details

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ASPsiRNA information siRNA Id:aspsirna0347
siRNA Name: ShAtaxMUT (C)
Mismatch information Wild siRNA (As strand 3'->5'):UCGUCGUCCCCCUGGAUAG
ASP-siRNA (As strand 3'->5'):UCGUCGUCGCCCUGGAUAG
Mismatch position in siRNA: G11C
Gene Information Gene Name Ataxin-3 (ATXN3)
Target Sequence (5'->3'):GCAGCAGCAGCAGCAGCAGCAGGGGGACCUAUC
Wild allele (5'->3'):AGCAGCAGGGGGACCUAUC
Mutant allele (5'->3'):AGCAGCAGCGGGACCUAUC
Position of siRNA on target gene: 977-995
Respective Gene/Protein Resources GenBank Accession:NM_004993.5
Cytogenic location:14q32.12  
Chromosomal coordinates:14:92,524,895-92,572,964
UniProt ID:P54252
HUGO ID:7106
Reference SNp(RefSNP): rs19392292
Disease/Mutation information Target Mutation: c.892_894CAG(8_36) (p.Gln298_Gln305=),c.987G>C
Matation type/variant: Microsatellite/Single nucleotide variant/Missense variant
Mutation at gene level: NG_008198.2:g.40588_40590CAG(8_36)
Pathogenic status of mutation: Pathogenic
Disease:Machado-Joseph disease (MJD)/Spinocerebellar ataxia type 3 (SCA3)
Clinical Resources ClinVar ID:3551
KEGG disease ID:H00063
OMIM ID: 109150
COSMIC: ATXN3
DECIPHER: ATXN3
GeneTests: ATXN3
ASP siRNA details Mutant allele (5'->3'): AGCAGCAGCGGGACCUAUC
ASP-siRNA (As strand 3'->5'):UCGUCGUCGCCCUGGAUAG
Percentage efficacy of ASP-siRNA for mutant allele:16
Wild allele (5'->3'): AGCAGCAGGGGGACCUAUC
ASP-siRNA (As strand 3'->5'):UCGUCGUCGCCCUGGAUAG
Percentage efficacy of ASP-siRNA for wild allele:0
Relative difference:16
Wild siRNA details Wild allele (5'->3'): AGCAGCAGGGGGACCUAUC
Wild siRNA (As strand 3'->5'):UCGUCGUCCCCCUGGAUAG
Percentage efficacy of wild siRNA for wild allele:17
Wild allele (5'->3'): AGCAGCAGGGGGACCUAUC
ASP-siRNA (As strand 3'->5'):UCGUCGUCGCCCUGGAUAG
Percentage efficacy of Wild sirna for mutant allele:20
Relative difference :-3
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: 293T
Experimental technique used: Western blot, RT-PCR
Transfection method: CaPO4 mediated transfection
siRNA expression method: Chemically synthesized
Post-transfection duration: 48 hours
Concentration used:5mg
Reference:18841197
Delivery method:Transfection
Article title:Allele-Specific RNA Silencing of Mutant Ataxin-3 Mediates Neuroprotection in a Rat Model of Machado-Joseph Disease
Authors:Alves S, Nascimento-Ferreira I, Auregan G, Hassig R, Dufour N, Brouillet E, Pedroso de Lima MC, Hantraye P, Pereira de Almeida L, Déglon N
Journal Reference:PLoS One. 2008 Oct 8;3(10):e3341. doi: 10.1371/journal.pone.0003341