aspsirna0437 details

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ASPsiRNA information siRNA Id:aspsirna0437
siRNA Name: P10
Mismatch information Wild siRNA (As strand 3'->5'):AUGUCACAAGACCAACCAU
ASP-siRNA (As strand 3'->5'):AUGUCACAACACCAACCAU
Mismatch position in siRNA: C10G
Gene Information Gene Name Presenilin1 (PSEN1)
Target Sequence (5'->3'):UUCAUUUUCUACAGUGUUCUGGUUGGUAAAGCCUCAG
Wild allele (5'->3'):UACAGUGUUCUGGUUGGUA
Mutant allele (5'->3'):UACAGUGUUGUGGUUGGUA
Position of siRNA on target gene: 1449-1467
Respective Gene/Protein Resources GenBank Accession:NM_000021.3
Cytogenic location:14q24.2
Chromosomal coordinates:14:73,603,142-73,690,398
UniProt ID:P49768
HUGO ID:9508
Reference SNp(RefSNP): rs63750886
Disease/Mutation information Target Mutation: c.811C>G, p.Leu271Val
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_007386.2:g.66602C>G
Pathogenic status of mutation: Pathogenic
Disease:Alzheimers disease
Clinical Resources ClinVar ID:98106
KEGG disease ID:H00056
OMIM ID: 104300
COSMIC: PSEN1
DECIPHER: PSEN1
GeneTests: PSEN1
ASP siRNA details Mutant allele (5'->3'): UACAGUGUUGUGGUUGGUA
ASP-siRNA (As strand 3'->5'):AUGUCACAACACCAACCAU
Percentage efficacy of ASP-siRNA for mutant allele:65
Wild allele (5'->3'): UACAGUGUUCUGGUUGGUA
ASP-siRNA (As strand 3'->5'):AUGUCACAACACCAACCAU
Percentage efficacy of ASP-siRNA for wild allele:7
Relative difference:58
Wild siRNA details Wild allele (5'->3'): UACAGUGUUCUGGUUGGUA
Wild siRNA (As strand 3'->5'):AUGUCACAAGACCAACCAU
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): UACAGUGUUCUGGUUGGUA
ASP-siRNA (As strand 3'->5'):AUGUCACAACACCAACCAU
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Chemically synthesized (Phosphoramidite method)
Post-transfection duration: 48 hours
Concentration used:1.5microL
Reference:21559198
Delivery method:Transfection
Article title:Specific Silencing of L392V PSEN1 Mutant Allele by RNA Interference.
Authors:Sierant M, Paduszynska A, Kazmierczak-Baranska J, Nacmias B, Sorbi S, Bagnoli S, Sochacka E, Nawrot B.
Journal Reference:Int J Alzheimers Dis. 2011 Apr 7;2011:809218. doi: 10.4061/2011/809218.