| | |
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| ASPsiRNA information |
siRNA Id: | aspsirna0524
|
|
siRNA Name: | B
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | NA
|
|
ASP-siRNA (As strand 3'->5'): | UUCCUGGUCAACCCGUUCUUA
|
|
Mismatch position in siRNA: | NA |
| Gene Information |
Gene Name | Synuclein, alpha (SNCA) |
|
Target Sequence (5'->3'): | CAGCCACTGGCTTTGTCAAAAAGGACCAG
|
|
Wild allele (5'->3'): | NA
|
|
Mutant allele (5'->3'): | AAGGACCAGUUGGGCAAGAAU
|
|
Position of siRNA on target gene: | 552-572
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000345.3 |
| Cytogenic location: | 4q22.1 |
| Chromosomal coordinates: | 4:90,645,249-90,759,446 |
| UniProt ID: | P37840 |
| HUGO ID: | 11138 |
|
Reference SNp(RefSNP): | rs10489387 |
| Disease/Mutation information |
Target Mutation: | c.157G>A,p.Ala53Thr
|
|
Matation type/variant: | Single nucleotide variant/Missense variant
|
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Mutation at gene level: | NG_011851.1:g.15148G>A
|
|
Pathogenic status of mutation: | Pathogenic
|
|
Disease: | Parkinsons disease (PD) |
| Clinical Resources |
ClinVar ID: | 14007 |
|
OMIM ID: | 168600 |
|
COSMIC: | SNCA |
|
DECIPHER: | SNCA |
|
GeneTests: | SNCA |
| ASP siRNA details |
Mutant allele (5'->3'): | AAGGACCAGUUGGGCAAGAAU
|
|
ASP-siRNA (As strand 3'->5'): | UUCCUGGUCAACCCGUUCUUA
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | NA
|
|
Wild allele (5'->3'): | NA
|
|
ASP-siRNA (As strand 3'->5'): | UUCCUGGUCAACCCGUUCUUA
|
|
Percentage efficacy of ASP-siRNA for wild allele: | NA
|
|
Relative difference: | NA
|
| Wild siRNA details |
Wild allele (5'->3'): | NA
|
|
Wild siRNA (As strand 3'->5'): | NA
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | NA
|
|
ASP-siRNA (As strand 3'->5'): | UUCCUGGUCAACCCGUUCUUA
|
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
|
Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA
|
| Cell-line used: | HeLa |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Qiagen–Xeragon |
| Post-transfection duration: | 48 hours |
| Concentration used: | 0.1nM |
| Reference: | 16455076 |
| Delivery method: | shRNA expression vector delivery |
| Article title: | Silencing of human alpha-synuclein in vitro and in rat brain using lentiviral-mediated RNAi. |
| Authors: | Sapru MK, Yates JW, Hogan S, Jiang L, Halter J, Bohn MC. |
| Journal Reference: | Exp Neurol. 2006 Apr;198(2):382-90. Epub 2006 Feb 7. |
