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| ASPsiRNA information |
siRNA Id: | aspsirna0537
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siRNA Name: | Si9,10
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | UACCACACCGUUGUCACCGAC
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ASP-siRNA (As strand 3'->5'): | UACCACACUCUUGUCACCGAC
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Mismatch position in siRNA: | U13C,C12G |
| Gene Information |
Gene Name | Synuclein, alpha (SNCA) |
|
Target Sequence (5'->3'): | GTGGTGCAUGGUGUGGCAACAGUGGCUG
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Wild allele (5'->3'): | AUGGUGUGGCAACAGUGGCUG
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Mutant allele (5'->3'): | AUGGUGUGAGAACAGUGGCUG
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Position of siRNA on target gene: | 412-432
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000345.3 |
| Cytogenic location: | 4q22.1 |
| Chromosomal coordinates: | 4:90,645,249-90,759,446 |
| UniProt ID: | P37840 |
| HUGO ID: | 11138 |
|
Reference SNp(RefSNP): | rs10489387 |
| Disease/Mutation information |
Target Mutation: | c.157G>A,p.Ala53Thr
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_011851.1:g.15148G>A
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Pathogenic status of mutation: | Pathogenic
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Disease: | Parkinsons disease (PD) |
| Clinical Resources |
ClinVar ID: | 14007 |
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KEGG disease ID: | H00057 |
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OMIM ID: | 168600 |
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COSMIC: | SNCA |
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DECIPHER: | SNCA |
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GeneTests: | SNCA |
| ASP siRNA details |
Mutant allele (5'->3'): | AUGGUGUGAGAACAGUGGCUG
|
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ASP-siRNA (As strand 3'->5'): | UACCACACUCUUGUCACCGAC
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 75
|
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Wild allele (5'->3'): | AUGGUGUGGCAACAGUGGCUG
|
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ASP-siRNA (As strand 3'->5'): | UACCACACUCUUGUCACCGAC
|
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Percentage efficacy of ASP-siRNA for wild allele: | 55
|
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Relative difference: | 20
|
| Wild siRNA details |
Wild allele (5'->3'): | AUGGUGUGGCAACAGUGGCUG
|
|
Wild siRNA (As strand 3'->5'): | UACCACACCGUUGUCACCGAC
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | AUGGUGUGGCAACAGUGGCUG
|
|
ASP-siRNA (As strand 3'->5'): | UACCACACUCUUGUCACCGAC
|
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
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Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | SH-SY5Y |
| Experimental technique used: | Western blot |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | QiagenXeragon |
| Post-transfection duration: | 72 hours |
| Concentration used: | 0.1nM |
| Reference: | 16455076 |
| Delivery method: | shRNA expression vector delivery |
'| Article title: | Silencing of human alpha-synuclein in vitro and in rat brain using lentiviral-mediated RNAi. |
'| Authors: | Sapru MK, Yates JW, Hogan S, Jiang L, Halter J, Bohn MC. |
'| Journal Reference: | Exp Neurol. 2006 Apr;198(2):382-90. Epub 2006 Feb 7. |
'
