aspsirna0537 details

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ASPsiRNA information siRNA Id:aspsirna0537
siRNA Name: Si9,10
Mismatch information Wild siRNA (As strand 3'->5'):UACCACACCGUUGUCACCGAC
ASP-siRNA (As strand 3'->5'):UACCACACUCUUGUCACCGAC
Mismatch position in siRNA: U13C,C12G
Gene Information Gene Name Synuclein, alpha (SNCA)
Target Sequence (5'->3'):GTGGTGCAUGGUGUGGCAACAGUGGCUG
Wild allele (5'->3'):AUGGUGUGGCAACAGUGGCUG
Mutant allele (5'->3'):AUGGUGUGAGAACAGUGGCUG
Position of siRNA on target gene: 412-432
Respective Gene/Protein Resources GenBank Accession:NM_000345.3
Cytogenic location:4q22.1
Chromosomal coordinates:4:90,645,249-90,759,446
UniProt ID:P37840
HUGO ID:11138
Reference SNp(RefSNP): rs10489387
Disease/Mutation information Target Mutation: c.157G>A,p.Ala53Thr
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_011851.1:g.15148G>A
Pathogenic status of mutation: Pathogenic
Disease:Parkinsons disease (PD)
Clinical Resources ClinVar ID:14007
KEGG disease ID:H00057
OMIM ID: 168600
COSMIC: SNCA
DECIPHER: SNCA
GeneTests: SNCA
ASP siRNA details Mutant allele (5'->3'): AUGGUGUGAGAACAGUGGCUG
ASP-siRNA (As strand 3'->5'):UACCACACUCUUGUCACCGAC
Percentage efficacy of ASP-siRNA for mutant allele:75
Wild allele (5'->3'): AUGGUGUGGCAACAGUGGCUG
ASP-siRNA (As strand 3'->5'):UACCACACUCUUGUCACCGAC
Percentage efficacy of ASP-siRNA for wild allele:55
Relative difference:20
Wild siRNA details Wild allele (5'->3'): AUGGUGUGGCAACAGUGGCUG
Wild siRNA (As strand 3'->5'):UACCACACCGUUGUCACCGAC
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): AUGGUGUGGCAACAGUGGCUG
ASP-siRNA (As strand 3'->5'):UACCACACUCUUGUCACCGAC
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: SH-SY5Y
Experimental technique used: Western blot
Transfection method: Lipofectamine 2000
siRNA expression method: Qiagen–Xeragon
Post-transfection duration: 72 hours
Concentration used:0.1nM
Reference:16455076
Delivery method:shRNA expression vector delivery
Article title:Silencing of human alpha-synuclein in vitro and in rat brain using lentiviral-mediated RNAi.
Authors:Sapru MK, Yates JW, Hogan S, Jiang L, Halter J, Bohn MC.
Journal Reference:Exp Neurol. 2006 Apr;198(2):382-90. Epub 2006 Feb 7.