aspsirna0542 details

''''
ASPsiRNA information siRNA Id:aspsirna0542
siRNA Name: SM2-RG(11)
Mismatch information Wild siRNA (As strand 3'->5'):AUUUCCGAGCGCGAAGAAG
ASP-siRNA (As strand 3'->5'):AUUUCCGACCGCGAAGAAG
Mismatch position in siRNA: C11G
Gene Information Gene Name Leucine rich repeat kinase 2 (LRRK2)
Target Sequence (5'->3'):CCTTGGCTCTTCAATATAAAGGCTCGCGCTTCTTCTTCCCCTG
Wild allele (5'->3'):UAAAGGCUCGCGCUUCUUC
Mutant allele (5'->3'):UAAAGGCUGGCGCUUCUUC
Position of siRNA on target gene: 4434-4452
Respective Gene/Protein Resources GenBank Accession:NM_198578.3
Cytogenic location:12q12
Chromosomal coordinates:12:40,618,812-40,763,086
UniProt ID:Q5S007
HUGO ID:18618
Reference SNp(RefSNP): rs33939927
Disease/Mutation information Target Mutation: c.4321C>G,p.Arg1441Gly
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_011709.1:g.90424C>G
Pathogenic status of mutation: Pathogenic
Disease:Parkinsons disease (PD)
Clinical Resources ClinVar ID:1936
KEGG disease ID:H00057
OMIM ID: 168600
COSMIC: LRRK2
DECIPHER: LRRK2
GeneTests: LRRK2
ASP siRNA details Mutant allele (5'->3'): UAAAGGCUGGCGCUUCUUC
ASP-siRNA (As strand 3'->5'):AUUUCCGACCGCGAAGAAG
Percentage efficacy of ASP-siRNA for mutant allele:20
Wild allele (5'->3'): UAAAGGCUCGCGCUUCUUC
ASP-siRNA (As strand 3'->5'):AUUUCCGACCGCGAAGAAG
Percentage efficacy of ASP-siRNA for wild allele:6
Relative difference:14
Wild siRNA details Wild allele (5'->3'): UAAAGGCUCGCGCUUCUUC
Wild siRNA (As strand 3'->5'):AUUUCCGAGCGCGAAGAAG
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): UAAAGGCUCGCGCUUCUUC
ASP-siRNA (As strand 3'->5'):AUUUCCGACCGCGAAGAAG
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: 293T
Experimental technique used: Dual luciferase reporter assay
Transfection method: Arrest-In reagent
siRNA expression method: Open-Biosystems
Post-transfection duration: 48 hours
Concentration used:400ng
Reference:21712955
Delivery method:Transfection
Article title:Efficient allele-specific targeting of LRRK2 R1441 mutations mediated by RNAi.
Authors:de Yńigo-Mojado L, Martín-Ruíz I, Sutherland JD.
Journal Reference:PLoS One. 2011;6(6):e21352. doi: 10.1371/journal.pone.0021352. Epub 2011 Jun 21.