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| ASPsiRNA information |
siRNA Id: | aspsirna0543
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siRNA Name: | SM2-RC(11)
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | AUUUCCGAGCGCGAAGAAG
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ASP-siRNA (As strand 3'->5'): | AUUUCCGAACGCGAAGAAG
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Mismatch position in siRNA: | A11G |
| Gene Information |
Gene Name | Leucine rich repeat kinase 2 (LRRK2) |
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Target Sequence (5'->3'): | CCTTGGCTCTTCAATATAAAGGCTCGCGCTTCTTCTTCCCCTG
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Wild allele (5'->3'): | UAAAGGCUCGCGCUUCUUC
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Mutant allele (5'->3'): | UAAAGGCUUGCGCUUCUUC
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Position of siRNA on target gene: | 4434-4452
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| Respective Gene/Protein Resources |
GenBank Accession: | NM_198578.3 |
| Cytogenic location: | 12q12 |
| Chromosomal coordinates: | 12:40,618,812-40,763,086 |
| UniProt ID: | Q5S007 |
| HUGO ID: | 18618 |
|
Reference SNp(RefSNP): | rs33939927 |
| Disease/Mutation information |
Target Mutation: | c.4321C>T,p.Arg1441Cys
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_011709.1:g.90424C>T
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Pathogenic status of mutation: | Pathogenic
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Disease: | Parkinsons disease (PD) |
| Clinical Resources |
ClinVar ID: | 1938 |
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KEGG disease ID: | H00057 |
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OMIM ID: | 168600 |
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COSMIC: | LRRK2 |
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DECIPHER: | LRRK2 |
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GeneTests: | LRRK2 |
| ASP siRNA details |
Mutant allele (5'->3'): | UAAAGGCUUGCGCUUCUUC
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ASP-siRNA (As strand 3'->5'): | AUUUCCGAACGCGAAGAAG
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 63
|
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Wild allele (5'->3'): | UAAAGGCUCGCGCUUCUUC
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ASP-siRNA (As strand 3'->5'): | AUUUCCGAACGCGAAGAAG
|
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Percentage efficacy of ASP-siRNA for wild allele: | 75
|
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Relative difference: | -12
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| Wild siRNA details |
Wild allele (5'->3'): | UAAAGGCUCGCGCUUCUUC
|
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Wild siRNA (As strand 3'->5'): | AUUUCCGAGCGCGAAGAAG
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | UAAAGGCUCGCGCUUCUUC
|
|
ASP-siRNA (As strand 3'->5'): | AUUUCCGAACGCGAAGAAG
|
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Percentage efficacy of Wild sirna for mutant allele: | NA
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Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA
|
| Cell-line used: | 293T |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Arrest-In reagent |
| siRNA expression method: | Open-Biosystems |
| Post-transfection duration: | 48 hours |
| Concentration used: | 400ng |
| Reference: | 21712955 |
| Delivery method: | Transfection |
| Article title: | Efficient allele-specific targeting of LRRK2 R1441 mutations mediated by RNAi. |
| Authors: | de Yñigo-Mojado L, Martín-Ruíz I, Sutherland JD. |
| Journal Reference: | PLoS One. 2011;6(6):e21352. doi: 10.1371/journal.pone.0021352. Epub 2011 Jun 21. |
