aspsirna0555 details

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ASPsiRNA information siRNA Id:aspsirna0555
siRNA Name: siRNA7
Mismatch information Wild siRNA (As strand 3'->5'):AAACGACGAGUCAUACUA
ASP-siRNA (As strand 3'->5'):AAACGACGAGUCAUACUG
Mismatch position in siRNA: G1A
Gene Information Gene Name Collagen type I alpha 2 (COL1A2)
Target Sequence (5'->3'):TGGGAACTTTGCTGCTCAGTATGATGGAAAAGGAGTTGGACTT
Wild allele (5'->3'):UUUGCUGCUCAGUAUGAU
Mutant allele (5'->3'):UUUGCUGCUCAGUAUGAC
Position of siRNA on target gene: 700-717
Respective Gene/Protein Resources GenBank Accession:NM_000089.3
Cytogenic location:7q21.3
Chromosomal coordinates:7:94,023,872-94,060,543
UniProt ID:P08123
HUGO ID:2198
Reference SNp(RefSNP): rs1800222
Disease/Mutation information Target Mutation: c.246T>C,NP_000080.2:p.Asp82=
Mutation at gene level: NG_007405.1:g.12027T>C
Pathogenic status of mutation: Disease causing
Disease:Osteogenesis Imperfecta (OI)
Clinical Resources ClinVar ID:NA
KEGG disease ID:H00506
OMIM ID: 166210
COSMIC: COL1A2
DECIPHER: COL1A2
GeneTests: COL1A2
ASP siRNA details Mutant allele (5'->3'): UUUGCUGCUCAGUAUGAC
ASP-siRNA (As strand 3'->5'):AAACGACGAGUCAUACUG
Percentage efficacy of ASP-siRNA for mutant allele:83
Wild allele (5'->3'): UUUGCUGCUCAGUAUGAU
ASP-siRNA (As strand 3'->5'):AAACGACGAGUCAUACUG
Percentage efficacy of ASP-siRNA for wild allele:100
Relative difference:-17
Wild siRNA details Wild allele (5'->3'): UUUGCUGCUCAGUAUGAU
Wild siRNA (As strand 3'->5'):AAACGACGAGUCAUACUA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): UUUGCUGCUCAGUAUGAU
ASP-siRNA (As strand 3'->5'):AAACGACGAGUCAUACUG
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Primary human bone
Experimental technique used: RT-PCR
Transfection method: Magnet asisted transfection (MATRA)
siRNA expression method: Ambion
Post-transfection duration: 48 hours
Concentration used:0.6microg
Reference:19015742
Delivery method:Transfection
Article title:Allele dependent silencing of COL1A2 using small interfering RNAs.
Authors:Lindahl K, Rubin CJ, Kindmark A, Ljunggren O.
Journal Reference:Int J Med Sci. 2008;5(6):361-5. Epub 2008 Nov 12.