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| ASPsiRNA information |
siRNA Id: | aspsirna0563
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|
siRNA Name: | R M10.11-shRNA
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CUCUUGCAGUAGUGGCUCAA
|
|
ASP-siRNA (As strand 3'->5'): | CUCUUGCAGCGGUGGCUCAA
|
|
Mismatch position in siRNA: | G10A,C11U |
| Gene Information |
Gene Name | DsRed2 |
|
Target Sequence (5'->3'): | GGATCCTGATGGCCTCCTCCGAGAACGTC
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|
Wild allele (5'->3'): | GAGAACGUCAUCACCGAGUU
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Mutant allele (5'->3'): | GAGAACGUCGCCACCGAGUU
|
|
Position of siRNA on target gene: | 1392-1411
|
| Respective Gene/Protein Resources |
GenBank Accession: | DQ005468.1 |
| Cytogenic location: | NA |
| Chromosomal coordinates: | NA |
| UniProt ID: | Q2WG74 |
| HUGO ID: | NA |
|
Reference SNp(RefSNP): | NA |
| Disease/Mutation information |
Target Mutation: | NA
|
|
Matation type/variant: | NA
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|
Mutation at gene level: | NA
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|
Pathogenic status of mutation: | Not provided
|
|
Disease: | Congenital myasthenic syndrome (SCCMS) |
| Clinical Resources |
ClinVar ID: | NA |
|
KEGG disease ID: | H00770 |
|
OMIM ID: | 608931 |
|
COSMIC: | DsRed2 |
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DECIPHER: | DsRed2 |
|
GeneTests: | DsRed2 |
| ASP siRNA details |
Mutant allele (5'->3'): | GAGAACGUCGCCACCGAGUU
|
|
ASP-siRNA (As strand 3'->5'): | CUCUUGCAGCGGUGGCUCAA
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 8
|
|
Wild allele (5'->3'): | GAGAACGUCAUCACCGAGUU
|
|
ASP-siRNA (As strand 3'->5'): | CUCUUGCAGCGGUGGCUCAA
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 25
|
|
Relative difference: | -17
|
| Wild siRNA details |
Wild allele (5'->3'): | GAGAACGUCAUCACCGAGUU
|
|
Wild siRNA (As strand 3'->5'): | CUCUUGCAGUAGUGGCUCAA
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | GAGAACGUCAUCACCGAGUU
|
|
ASP-siRNA (As strand 3'->5'): | CUCUUGCAGCGGUGGCUCAA
|
|
Percentage efficacy of Wild sirna for mutant allele: | 4
|
|
Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Flourescence microscopy |
| Transfection method: | Polyethyleneimine |
| siRNA expression method: | Dharmacon |
| Post-transfection duration: | 120 hours |
| Concentration used: | 100pmol |
| Reference: | 12928480 |
| Delivery method: | Transfection |
'| Article title: | Allele-specific silencing of a pathogenic mutant acetylcholine receptor subunit by RNA interference. |
'| Authors: | Abdelgany A, Wood M, Beeson D. |
'| Journal Reference: | Hum Mol Genet. 2003 Oct 15;12(20):2637-44. Epub 2003 Aug 19. |
'
