| | |
ASPsiRNA information |
siRNA Id: | aspsirna0565
|
siRNA Name: | SM1
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | UUCUACCUACGUCUUAAGGCU
|
ASP-siRNA (As strand 3'->5'): | UUAGACCUACGUCUUAAGGCU
|
Mismatch position in siRNA: | A3C,G4U |
Gene Information |
Gene Name | Amyloid beta (A4) precursor protein(APP) |
Target Sequence (5'->3'): | AAGUGAAGAUGG AUGCAGAAU
|
Wild allele (5'->3'): | AAGAUGGAUGCAGAAUUCCGA
|
Mutant allele (5'->3'): | AAUCUGGAUGCAGAAUUCCGA
|
Position of siRNA on target gene: | 2208-2228
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_000484.3 |
Cytogenic location: | 21q21.3 |
Chromosomal coordinates: | 21:27,252,860-27,543,445 |
UniProt ID: | P56817 |
HUGO ID: | 620 |
Reference SNp(RefSNP): | rs28186516 |
Disease/Mutation information |
Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
|
Matation type/variant: | Single nucleotide variant/Missense variant
|
Mutation at gene level: | NG_007376.1:g.278194_278195delGAinsTC
|
Pathogenic status of mutation: | Disease causing
|
Disease: | Alzheimers disease |
Clinical Resources |
ClinVar ID: | 18093 |
KEGG disease ID: | H00056 |
OMIM ID: | 104300 |
COSMIC: | APP |
DECIPHER: | APP |
GeneTests: | APP |
ASP siRNA details |
Mutant allele (5'->3'): | AAUCUGGAUGCAGAAUUCCGA
|
ASP-siRNA (As strand 3'->5'): | UUAGACCUACGUCUUAAGGCU
|
Percentage efficacy of ASP-siRNA for mutant allele: | 0
|
Wild allele (5'->3'): | AAGAUGGAUGCAGAAUUCCGA
|
ASP-siRNA (As strand 3'->5'): | UUAGACCUACGUCUUAAGGCU
|
Percentage efficacy of ASP-siRNA for wild allele: | 0
|
Relative difference: | High discrimination
|
Wild siRNA details |
Wild allele (5'->3'): | AAGAUGGAUGCAGAAUUCCGA
|
Wild siRNA (As strand 3'->5'): | UUCUACCUACGUCUUAAGGCU
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
Wild allele (5'->3'): | AAGAUGGAUGCAGAAUUCCGA
|
ASP-siRNA (As strand 3'->5'): | UUAGACCUACGUCUUAAGGCU
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
Relative difference : | NA
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | CHO |
Experimental technique used: | RT-PCR |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Epicentre Technologies |
Post-transfection duration: | 48 hours |
Concentration used: | 100pmol |
Reference: | 16426772 |
Delivery method: | Transfection |
'Article title: | Allele-specific silencing of Alzheimer's disease genes: the amyloid precursor protein genes with Swedish or London mutations. |
'Authors: | Feng X, Zhao P, He Y, Zuo Z. |
'Journal Reference: | Gene. 2006 Apr 12;371(1):68-74. Epub 2006 Jan 19. |
'