aspsirna0566 details

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ASPsiRNA information siRNA Id:aspsirna0566
siRNA Name: SM2
Mismatch information Wild siRNA (As strand 3'->5'):UUCACUUCUACCUACGUCUUA
ASP-siRNA (As strand 3'->5'):UUCACUUAGACCUACGUCUUA
Mismatch position in siRNA: A8C,G9U
Gene Information Gene Name Amyloid beta (A4) precursor protein(APP)
Target Sequence (5'->3'):AAGUGAAGAUGG AUGCAGAAU
Wild allele (5'->3'):AAGUGAAGAUGGAUGCAGAAU
Mutant allele (5'->3'):AAGUGAAUCUGGAUGCAGAAU
Position of siRNA on target gene: 2203-2223
Respective Gene/Protein Resources GenBank Accession:NM_000484.3
Cytogenic location:21q21.3
Chromosomal coordinates:21:27,252,860-27,543,445
UniProt ID:P56817
HUGO ID:620
Reference SNp(RefSNP): rs28186516
Disease/Mutation information Target Mutation: c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_007376.1:g.278194_278195delGAinsTC
Pathogenic status of mutation: Disease causing
Disease:Alzheimers disease
Clinical Resources ClinVar ID:18093
KEGG disease ID:H00056
OMIM ID: 104300
COSMIC: APP
DECIPHER: APP
GeneTests: APP
ASP siRNA details Mutant allele (5'->3'): AAGUGAAUCUGGAUGCAGAAU
ASP-siRNA (As strand 3'->5'):UUCACUUAGACCUACGUCUUA
Percentage efficacy of ASP-siRNA for mutant allele:0
Wild allele (5'->3'): AAGUGAAGAUGGAUGCAGAAU
ASP-siRNA (As strand 3'->5'):UUCACUUAGACCUACGUCUUA
Percentage efficacy of ASP-siRNA for wild allele:3
Relative difference:-3
Wild siRNA details Wild allele (5'->3'): AAGUGAAGAUGGAUGCAGAAU
Wild siRNA (As strand 3'->5'):UUCACUUCUACCUACGUCUUA
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): AAGUGAAGAUGGAUGCAGAAU
ASP-siRNA (As strand 3'->5'):UUCACUUAGACCUACGUCUUA
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: CHO
Experimental technique used: RT-PCR
Transfection method: Lipofectamine 2000
siRNA expression method: Epicentre Technologies
Post-transfection duration: 48 hours
Concentration used:100pmol
Reference:16426772
Delivery method:Transfection
Article title:Allele-specific silencing of Alzheimer's disease genes: the amyloid precursor protein genes with Swedish or London mutations.
Authors:Feng X, Zhao P, He Y, Zuo Z.
Journal Reference:Gene. 2006 Apr 12;371(1):68-74. Epub 2006 Jan 19.