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| ASPsiRNA information |
siRNA Id: | aspsirna0567
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|
siRNA Name: | SM3
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | ACUUCACUUCUACCUACGU
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUAGACCUACGU
|
|
Mismatch position in siRNA: | A10C,G11U |
| Gene Information |
Gene Name | Amyloid beta (A4) precursor protein(APP) |
|
Target Sequence (5'->3'): | AAGUGAAGAUGG AUGCAGAAU
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|
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCA
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Mutant allele (5'->3'): | UGAAGUGAAUCUGGAUGCA
|
|
Position of siRNA on target gene: | 2201-2219
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000484.3 |
| Cytogenic location: | 21q21.3 |
| Chromosomal coordinates: | 21:27,252,860-27,543,445 |
| UniProt ID: | P56817 |
| HUGO ID: | 620 |
|
Reference SNp(RefSNP): | rs28186516 |
| Disease/Mutation information |
Target Mutation: | c.2010_2011delGAinsTC (p.Lys670_Met671delinsAsnLeu)
|
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Matation type/variant: | Single nucleotide variant/Missense variant
|
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Mutation at gene level: | NG_007376.1:g.278194_278195delGAinsTC
|
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Pathogenic status of mutation: | Disease causing
|
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Disease: | Alzheimers disease |
| Clinical Resources |
ClinVar ID: | 18093 |
|
KEGG disease ID: | H00056 |
|
OMIM ID: | 104300 |
|
COSMIC: | APP |
|
DECIPHER: | APP |
|
GeneTests: | APP |
| ASP siRNA details |
Mutant allele (5'->3'): | UGAAGUGAAUCUGGAUGCA
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUAGACCUACGU
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 3
|
|
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCA
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUAGACCUACGU
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 70
|
|
Relative difference: | -67
|
| Wild siRNA details |
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCA
|
|
Wild siRNA (As strand 3'->5'): | ACUUCACUUCUACCUACGU
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | UGAAGUGAAGAUGGAUGCA
|
|
ASP-siRNA (As strand 3'->5'): | ACUUCACUUAGACCUACGU
|
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
|
Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | CHO |
| Experimental technique used: | RT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Epicentre Technologies |
| Post-transfection duration: | 48 hours |
| Concentration used: | 100pmol |
| Reference: | 16426772 |
| Delivery method: | Transfection |
'| Article title: | Allele-specific silencing of Alzheimer's disease genes: the amyloid precursor protein genes with Swedish or London mutations. |
'| Authors: | Feng X, Zhao P, He Y, Zuo Z. |
'| Journal Reference: | Gene. 2006 Apr 12;371(1):68-74. Epub 2006 Jan 19. |
'
