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| ASPsiRNA information |
siRNA Id: | aspsirna0574
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siRNA Name: | LM2C
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | GCUGUCACUAGCAGUAGUGGAA
|
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ASP-siRNA (As strand 3'->5'): | CCUGUCAGUAGUAGUAGUGGAA
|
|
Mismatch position in siRNA: | U11C,G15C,C22G |
| Gene Information |
Gene Name | Amyloid beta (A4) precursor protein(APP) |
|
Target Sequence (5'->3'): | AGCGACAGUGAUCGUCAUCACCU
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Wild allele (5'->3'): | CGACAGUGAUCGUCAUCACCUU
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Mutant allele (5'->3'): | GGACAGUCAUCAUCAUCACCUU
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|
Position of siRNA on target gene: | 2338-2359
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000484.3 |
| Cytogenic location: | 21q21.3 |
| Chromosomal coordinates: | 21:27,252,860-27,543,445 |
| UniProt ID: | P05067 |
| HUGO ID: | 620 |
|
Reference SNp(RefSNP): | rs63750264 |
| Disease/Mutation information |
Target Mutation: | c.2149G>A,p.Val717Ile
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_007376.1:g.284037G>A
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Pathogenic status of mutation: | Disease causing
|
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Disease: | Alzheimers disease |
| Clinical Resources |
ClinVar ID: | 18088 |
|
KEGG disease ID: | H00056 |
|
OMIM ID: | 104300 |
|
COSMIC: | APP |
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DECIPHER: | APP |
|
GeneTests: | APP |
| ASP siRNA details |
Mutant allele (5'->3'): | GGACAGUCAUCAUCAUCACCUU
|
|
ASP-siRNA (As strand 3'->5'): | CCUGUCAGUAGUAGUAGUGGAA
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 29
|
|
Wild allele (5'->3'): | CGACAGUGAUCGUCAUCACCUU
|
|
ASP-siRNA (As strand 3'->5'): | CCUGUCAGUAGUAGUAGUGGAA
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 75
|
|
Relative difference: | -46
|
| Wild siRNA details |
Wild allele (5'->3'): | CGACAGUGAUCGUCAUCACCUU
|
|
Wild siRNA (As strand 3'->5'): | GCUGUCACUAGCAGUAGUGGAA
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | CGACAGUGAUCGUCAUCACCUU
|
|
ASP-siRNA (As strand 3'->5'): | CCUGUCAGUAGUAGUAGUGGAA
|
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
|
Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | CHO |
| Experimental technique used: | RT-PCR |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Epicentre Technologies |
| Post-transfection duration: | 48 hours |
| Concentration used: | 100pmol |
| Reference: | 16426772 |
| Delivery method: | Transfection |
'| Article title: | Allele-specific silencing of Alzheimer's disease genes: the amyloid precursor protein genes with Swedish or London mutations. |
'| Authors: | Feng X, Zhao P, He Y, Zuo Z. |
'| Journal Reference: | Gene. 2006 Apr 12;371(1):68-74. Epub 2006 Jan 19. |
'
