aspsirna0657 details

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ASPsiRNA information siRNA Id:aspsirna0657
siRNA Name: P9
Mismatch information Wild siRNA (As strand 3'->5'):GCUGCUGCUGCUGCUGCUG
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGACGUCGUCG
Mismatch position in siRNA: A9U
Gene Information Gene Name Hungtintin (HTT)
Target Sequence (5'->3'):CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
Wild allele (5'->3'):CGACGACGACGACGACGAC
Mutant allele (5'->3'):CAGCAGCAGCUGCAGCAGC
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NM_002111.7
Cytogenic location:4p16.3
Chromosomal coordinates:4:3,076,407-3,245,686
UniProt ID:Q96CV9
HUGO ID:4851
Reference SNp(RefSNP): rs19392295
Disease/Mutation information Target Mutation: c.52CAG(36_39)
Matation type/variant: Microsatellite
Mutation at gene level: NG_009378.1:g.5197CAG(36_39)
Pathogenic status of mutation: Pathogenic
Disease:Huntington disease (HD)
Clinical Resources ClinVar ID:31916
KEGG disease ID:H01243
OMIM ID: 143100
COSMIC: HTT
DECIPHER: HTT
GeneTests: HTT
ASP siRNA details Mutant allele (5'->3'): CAGCAGCAGCUGCAGCAGC
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGACGUCGUCG
Percentage efficacy of ASP-siRNA for mutant allele:30
Wild allele (5'->3'): CGACGACGACGACGACGAC
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGACGUCGUCG
Percentage efficacy of ASP-siRNA for wild allele:20
Relative difference:10
Wild siRNA details Wild allele (5'->3'): CGACGACGACGACGACGAC
Wild siRNA (As strand 3'->5'):GCUGCUGCUGCUGCUGCUG
Percentage efficacy of wild siRNA for wild allele:Lowest effective
Wild allele (5'->3'): CGACGACGACGACGACGAC
ASP-siRNA (As strand 3'->5'):GUCGUCGUCGACGUCGUCG
Percentage efficacy of Wild sirna for mutant allele:65
Relative difference :discriminative
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Fibroblast
Experimental technique used: Western blot
Transfection method: RNAiMAX
siRNA expression method: Integrated DNA Technologies
Post-transfection duration: 72 hours
Concentration used:50nM
Reference:23042244
Delivery method:Transfection
Article title:Mechanism of allele-selective inhibition of huntingtin expression by duplex RNAs that target CAG repeats: function through the RNAi pathway.
Authors:Hu J, Liu J, Yu D, Chu Y, Corey DR.
Journal Reference:Nucleic Acids Res. 2012 Dec;40(22):11270-80. doi: 10.1093/nar/gks907. Epub 2012 Oct 4.