aspsirna0678 details

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ASPsiRNA information siRNA Id:aspsirna0678
siRNA Name: P10R
Mismatch information Wild siRNA (As strand 3'->5'):GUCGUCGUCGUCGUCGUCGUCG
ASP-siRNA (As strand 3'->5'):GUCGUCGUCAUCGUCGUCGUCG
Mismatch position in siRNA: A13G
Gene Information Gene Name Hungtintin (HTT)
Target Sequence (5'->3'):CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
Wild allele (5'->3'):CAGCAGCAGCAGCAGCAGCAGC
Mutant allele (5'->3'):CAGCAGCAGUAGCAGCAGCAGC
Position of siRNA on target gene: 368-389
Respective Gene/Protein Resources GenBank Accession:NM_002111.7
Cytogenic location:4p16.3
Chromosomal coordinates:4:3,076,407-3,245,686
UniProt ID:Q96CV9
HUGO ID:4851
Reference SNp(RefSNP): rs19392295
Disease/Mutation information Target Mutation: c.52CAG(36_39)
Matation type/variant: Microsatellite
Mutation at gene level: NG_009378.1:g.5197CAG(36_39)
Pathogenic status of mutation: Pathogenic
Disease:Huntington disease (HD)
Clinical Resources ClinVar ID:31916
KEGG disease ID:H00059
OMIM ID: 143100
COSMIC: HTT
DECIPHER: HTT
GeneTests: HTT
ASP siRNA details Mutant allele (5'->3'): CAGCAGCAGUAGCAGCAGCAGC
ASP-siRNA (As strand 3'->5'):GUCGUCGUCAUCGUCGUCGUCG
Percentage efficacy of ASP-siRNA for mutant allele:46
Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGCAGC
ASP-siRNA (As strand 3'->5'):GUCGUCGUCAUCGUCGUCGUCG
Percentage efficacy of ASP-siRNA for wild allele:40
Relative difference:6
Wild siRNA details Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGCAGC
Wild siRNA (As strand 3'->5'):GUCGUCGUCGUCGUCGUCGUCG
Percentage efficacy of wild siRNA for wild allele:Moderately effective
Wild allele (5'->3'): CAGCAGCAGCAGCAGCAGCAGC
ASP-siRNA (As strand 3'->5'):GUCGUCGUCAUCGUCGUCGUCG
Percentage efficacy of Wild sirna for mutant allele:20
Relative difference :High discrimination
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Fibroblast
Experimental technique used: Western blot
Transfection method: RNAiMAX
siRNA expression method: Isis Pharmaceuticals
Post-transfection duration: 4 days
Concentration used:3microliter
Reference:22939619
Delivery method:Transfection
Article title:Single-stranded RNAs use RNAi to potently and allele-selectively inhibit mutant huntingtin expression.
Authors:Yu D, Pendergraff H, Liu J, Kordasiewicz HB, Cleveland DW, Swayze EE, Lima WF, Crooke ST, Prakash TP, Corey DR.
Journal Reference:Cell. 2012 Aug 31;150(5):895-908. doi: 10.1016/j.cell.2012.08.002.