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| ASPsiRNA information |
siRNA Id: | aspsirna0678
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siRNA Name: | P10R
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUCGUCGUCG
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ASP-siRNA (As strand 3'->5'): | GUCGUCGUCAUCGUCGUCGUCG
|
|
Mismatch position in siRNA: | A13G |
| Gene Information |
Gene Name | Hungtintin (HTT) |
|
Target Sequence (5'->3'): | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAGC
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Mutant allele (5'->3'): | CAGCAGCAGUAGCAGCAGCAGC
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Position of siRNA on target gene: | 368-389
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_002111.7 |
| Cytogenic location: | 4p16.3 |
| Chromosomal coordinates: | 4:3,076,407-3,245,686 |
| UniProt ID: | Q96CV9 |
| HUGO ID: | 4851 |
|
Reference SNp(RefSNP): | rs19392295 |
| Disease/Mutation information |
Target Mutation: | c.52CAG(36_39)
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Matation type/variant: | Microsatellite
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Mutation at gene level: | NG_009378.1:g.5197CAG(36_39)
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Pathogenic status of mutation: | Pathogenic
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Disease: | Huntington disease (HD) |
| Clinical Resources |
ClinVar ID: | 31916 |
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KEGG disease ID: | H00059 |
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OMIM ID: | 143100 |
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COSMIC: | HTT |
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DECIPHER: | HTT |
|
GeneTests: | HTT |
| ASP siRNA details |
Mutant allele (5'->3'): | CAGCAGCAGUAGCAGCAGCAGC
|
|
ASP-siRNA (As strand 3'->5'): | GUCGUCGUCAUCGUCGUCGUCG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 46
|
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Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAGC
|
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ASP-siRNA (As strand 3'->5'): | GUCGUCGUCAUCGUCGUCGUCG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 40
|
|
Relative difference: | 6
|
| Wild siRNA details |
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAGC
|
|
Wild siRNA (As strand 3'->5'): | GUCGUCGUCGUCGUCGUCGUCG
|
|
Percentage efficacy of wild siRNA for wild allele: | Moderately effective
|
|
Wild allele (5'->3'): | CAGCAGCAGCAGCAGCAGCAGC
|
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ASP-siRNA (As strand 3'->5'): | GUCGUCGUCAUCGUCGUCGUCG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 20
|
|
Relative difference : | High discrimination
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | Fibroblast |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiMAX |
| siRNA expression method: | Isis Pharmaceuticals |
| Post-transfection duration: | 4 days |
| Concentration used: | 3microliter |
| Reference: | 22939619 |
| Delivery method: | Transfection |
'| Article title: | Single-stranded RNAs use RNAi to potently and allele-selectively inhibit mutant huntingtin expression. |
'| Authors: | Yu D, Pendergraff H, Liu J, Kordasiewicz HB, Cleveland DW, Swayze EE, Lima WF, Crooke ST, Prakash TP, Corey DR. |
'| Journal Reference: | Cell. 2012 Aug 31;150(5):895-908. doi: 10.1016/j.cell.2012.08.002. |
'
