| | |
ASPsiRNA information |
siRNA Id: | aspsirna0699
|
siRNA Name: | G10S
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | UCUAAACCUAGACCGUCAG
|
ASP-siRNA (As strand 3'->5'): | UCUAAACCUGGACCGUCAG
|
Mismatch position in siRNA: | G10A |
Gene Information |
Gene Name | Hungtintin (HTT) |
Target Sequence (5'->3'): | GCAAACCCUGACCGCAGUGCGGGGCAUUGG
|
Wild allele (5'->3'): | AGAUUUGGAUCUGGCAGUC
|
Mutant allele (5'->3'): | AGAUUUGGACCUGGCAGUC
|
Position of siRNA on target gene: | NA
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_002111.7 |
Cytogenic location: | 4p16.3 |
Chromosomal coordinates: | 4:3,076,407-3,245,686 |
UniProt ID: | Q96CV9 |
HUGO ID: | 4851 |
Reference SNp(RefSNP): | rs19392295 |
Disease/Mutation information |
Target Mutation: | c.52CAG(36_39)
|
Matation type/variant: | Microsatellite
|
Mutation at gene level: | NG_009378.1:g.5197CAG(36_39)
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Huntington disease (HD) |
Clinical Resources |
ClinVar ID: | 31916 |
KEGG disease ID: | H00059 |
OMIM ID: | 143100 |
COSMIC: | HTT |
DECIPHER: | HTT |
GeneTests: | HTT |
ASP siRNA details |
Mutant allele (5'->3'): | AGAUUUGGACCUGGCAGUC
|
ASP-siRNA (As strand 3'->5'): | UCUAAACCUGGACCGUCAG
|
Percentage efficacy of ASP-siRNA for mutant allele: | 75
|
Wild allele (5'->3'): | AGAUUUGGAUCUGGCAGUC
|
ASP-siRNA (As strand 3'->5'): | UCUAAACCUGGACCGUCAG
|
Percentage efficacy of ASP-siRNA for wild allele: | 25
|
Relative difference: | 50
|
Wild siRNA details |
Wild allele (5'->3'): | AGAUUUGGAUCUGGCAGUC
|
Wild siRNA (As strand 3'->5'): | UCUAAACCUAGACCGUCAG
|
Percentage efficacy of wild siRNA for wild allele: | 60
|
Wild allele (5'->3'): | AGAUUUGGAUCUGGCAGUC
|
ASP-siRNA (As strand 3'->5'): | UCUAAACCUGGACCGUCAG
|
Percentage efficacy of Wild sirna for mutant allele: | 15
|
Relative difference : | 45
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | Fibroblast |
Experimental technique used: | Western blot |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Metabion |
Post-transfection duration: | 72 hours |
Concentration used: | 50nM |
Reference: | 22397573 |
Delivery method: | Transfection |
'Article title: | An evaluation of oligonucleotide-based therapeutic strategies for polyQ diseases. |
'Authors: | Fiszer A, Olejniczak M, Switonski PM, Wroblewska JP, Wisniewska-Kruk J, Mykowska A, Krzyzosiak WJ. |
'Journal Reference: | BMC Mol Biol. 2012 Mar 7;13:6. doi: 10.1186/1471-2199-13-6. |
'