aspsirna0715 details

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ASPsiRNA information siRNA Id:aspsirna0715
siRNA Name: P11
Mismatch information Wild siRNA (As strand 3'->5'):CUCUGAACCCGUUACACUG
ASP-siRNA (As strand 3'->5'):CUCUGAACGCGUUACACUG
Mismatch position in siRNA: G11C
Gene Information Gene Name Cu,Zn superoxide dismutase (SOD1)
Target Sequence (5'->3'):TGAAGAGAGGCATGTTGGAGACTTGGGCAATGTGAC
Wild allele (5'->3'):GAGACUUGGGCAAUGUGAC
Mutant allele (5'->3'):GAGACUUGCGCAAUGUGAC
Position of siRNA on target gene: 396-414
Respective Gene/Protein Resources GenBank Accession:NM_000454.4
Cytogenic location:21q22.1
Chromosomal coordinates:21:33,031,934-33,041,243
UniProt ID:P00441
HUGO ID:11179
Reference SNp(RefSNP): rs12191243
Disease/Mutation information Target Mutation: c.256G>C, p.Gly86Arg
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_008689.1:g.12653G>C
Pathogenic status of mutation: Pathogenic
Disease:Amyotrophic Lateral Sclerosis (ALS)
Clinical Resources ClinVar ID:14758
KEGG disease ID:H00058
OMIM ID: 105400
COSMIC: SOD1
DECIPHER: SOD1
GeneTests: SOD1
ASP siRNA details Mutant allele (5'->3'): GAGACUUGCGCAAUGUGAC
ASP-siRNA (As strand 3'->5'):CUCUGAACGCGUUACACUG
Percentage efficacy of ASP-siRNA for mutant allele:76
Wild allele (5'->3'): GAGACUUGGGCAAUGUGAC
ASP-siRNA (As strand 3'->5'):CUCUGAACGCGUUACACUG
Percentage efficacy of ASP-siRNA for wild allele:25
Relative difference:51
Wild siRNA details Wild allele (5'->3'): GAGACUUGGGCAAUGUGAC
Wild siRNA (As strand 3'->5'):CUCUGAACCCGUUACACUG
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): GAGACUUGGGCAAUGUGAC
ASP-siRNA (As strand 3'->5'):CUCUGAACGCGUUACACUG
Percentage efficacy of Wild sirna for mutant allele:High efficacy
Relative difference :18
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Drosophila embryo lysate
Experimental technique used: Flourescence reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Chemically synthesized
Post-transfection duration: 24 hours
Concentration used:2nM
Reference:16965178
Delivery method:Transfection
Article title:Designing siRNA that distinguish between genes that differ by a single nucleotide.
Authors:Schwarz DS, Ding H, Kennington L, Moore JT, Schelter J, Burchard J, Linsley PS, Aronin N, Xu Z, Zamore PD.
Journal Reference:PLoS Genet. 2006 Sep 8;2(9):e140. Epub 2006 Jul 24.