aspsirna0771 details

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ASPsiRNA information siRNA Id:aspsirna0771
siRNA Name: P10A
Mismatch information Wild siRNA (As strand 3'->5'):CCUCUGAACCCGUUACACU
ASP-siRNA (As strand 3'->5'):ACCGAAAGUAAAUAUAACU
Mismatch position in siRNA: A10C
Gene Information Gene Name Cu,Zn superoxide dismutase (SOD1)
Target Sequence (5'->3'):TGAAGAGAGGCATGTTGGAGACTTGGGCAATGTGAC
Wild allele (5'->3'):GGAGACUUGGGCAAUGUGA
Mutant allele (5'->3'):UGGCUUUCAGUUAUAUUGA
Position of siRNA on target gene: 395-413
Respective Gene/Protein Resources GenBank Accession:NM_000454.4
Cytogenic location:21q22.1
Chromosomal coordinates:21:33,031,934-33,041,243
UniProt ID:P00441
HUGO ID:11179
Reference SNp(RefSNP): rs12191243
Disease/Mutation information Target Mutation: c.256G>C, p.Gly86Arg
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_008689.1:g.12653G>C
Pathogenic status of mutation: Pathogenic
Disease:Amyotrophic Lateral Sclerosis (ALS)
Clinical Resources ClinVar ID:14758
KEGG disease ID:H00058
OMIM ID: 105400
COSMIC: SOD1
DECIPHER: SOD1
GeneTests: SOD1
ASP siRNA details Mutant allele (5'->3'): UGGCUUUCAGUUAUAUUGA
ASP-siRNA (As strand 3'->5'):ACCGAAAGUAAAUAUAACU
Percentage efficacy of ASP-siRNA for mutant allele:87
Wild allele (5'->3'): GGAGACUUGGGCAAUGUGA
ASP-siRNA (As strand 3'->5'):ACCGAAAGUAAAUAUAACU
Percentage efficacy of ASP-siRNA for wild allele:23
Relative difference:64
Wild siRNA details Wild allele (5'->3'): GGAGACUUGGGCAAUGUGA
Wild siRNA (As strand 3'->5'):CCUCUGAACCCGUUACACU
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): GGAGACUUGGGCAAUGUGA
ASP-siRNA (As strand 3'->5'):ACCGAAAGUAAAUAUAACU
Percentage efficacy of Wild sirna for mutant allele:Moderate efficacy
Relative difference :45
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Flourescence reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Chemically synthesized
Post-transfection duration: 24 hours
Concentration used:2nM
Reference:16965178
Delivery method:Transfection
Article title:Designing siRNA that distinguish between genes that differ by a single nucleotide.
Authors:Schwarz DS, Ding H, Kennington L, Moore JT, Schelter J, Burchard J, Linsley PS, Aronin N, Xu Z, Zamore PD.
Journal Reference:PLoS Genet. 2006 Sep 8;2(9):e140. Epub 2006 Jul 24.