aspsirna0800 details

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ASPsiRNA information siRNA Id:aspsirna0800
siRNA Name: REPC-b
Mismatch information Wild siRNA (As strand 3'->5'):CGUCGUCGUCGUCGUCGUC
ASP-siRNA (As strand 3'->5'):CGUCGUCGUAGUCGUCGUC
Mismatch position in siRNA: A10C(as)
Gene Information Gene Name Hungtintin (HTT)
Target Sequence (5'->3'):CAGCAGCAGCAGCAGCAGCAGCAGCAGC
Wild allele (5'->3'):GCAGCAGCAGCAGCAGCAG
Mutant allele (5'->3'):GCAGCAGCAGCAGCAGCAG
Position of siRNA on target gene: 368-386
Respective Gene/Protein Resources GenBank Accession:NM_002111.7
Cytogenic location:4p16.3
Chromosomal coordinates:4:3,076,407-3,245,686
UniProt ID:Q96CV9
HUGO ID:4851
Reference SNp(RefSNP): rs19392295
Disease/Mutation information Target Mutation: c.52CAG(36_39)
Matation type/variant: Microsatellite
Mutation at gene level: NG_009378.1:g.5197CAG(36_39)
Pathogenic status of mutation: Pathogenic
Disease:Huntington disease (HD)
Clinical Resources ClinVar ID:31916
KEGG disease ID:H00059
OMIM ID: 143100
COSMIC: HTT
DECIPHER: HTT
GeneTests: HTT
ASP siRNA details Mutant allele (5'->3'): GCAGCAGCAGCAGCAGCAG
ASP-siRNA (As strand 3'->5'):CGUCGUCGUAGUCGUCGUC
Percentage efficacy of ASP-siRNA for mutant allele:84
Wild allele (5'->3'): GCAGCAGCAGCAGCAGCAG
ASP-siRNA (As strand 3'->5'):CGUCGUCGUAGUCGUCGUC
Percentage efficacy of ASP-siRNA for wild allele:15
Relative difference:69
Wild siRNA details Wild allele (5'->3'): GCAGCAGCAGCAGCAGCAG
Wild siRNA (As strand 3'->5'):CGUCGUCGUCGUCGUCGUC
Percentage efficacy of wild siRNA for wild allele:72
Wild allele (5'->3'): GCAGCAGCAGCAGCAGCAG
ASP-siRNA (As strand 3'->5'):CGUCGUCGUAGUCGUCGUC
Percentage efficacy of Wild sirna for mutant allele:50
Relative difference :22
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Fibroblast
Experimental technique used: Western blot
Transfection method: RNAiMAX
siRNA expression method: Integrated DNA Technologies
Post-transfection duration: 72 hours
Concentration used:25nM
Reference:21095568
Delivery method:Transfection
Article title:Allele-selective inhibition of huntingtin expression by switching to an miRNA-like RNAi mechanism.
Authors:Hu J, Liu J, Corey DR.
Journal Reference:Chem Biol. 2010 Nov 24;17(11):1183-8. doi: 10.1016/j.chembiol.2010.10.013.