aspsirna0805 details

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ASPsiRNA information siRNA Id:aspsirna0805
siRNA Name: SHP2
Mismatch information Wild siRNA (As strand 3'->5'):ACCUAAGGUACAAGUACUC
ASP-siRNA (As strand 3'->5'):AGCUAAGGUACAAGUACUC
Mismatch position in siRNA: G18C
Gene Information Gene Name Cu,Zn superoxide dismutase (SOD1)
Target Sequence (5'->3'):GGATTCCATGTTCATGAGTTTGGAGATAA
Wild allele (5'->3'):UGGAUUCCAUGUUCAUGAG
Mutant allele (5'->3'):UCGAUUCCAUGUUCAUGAG
Position of siRNA on target gene: 280-298
Respective Gene/Protein Resources GenBank Accession:NM_000454.4
Cytogenic location:21q22.1
Chromosomal coordinates:21:33,031,934-33,041,243
UniProt ID:P00441
HUGO ID:11179
Reference SNp(RefSNP): rs12191243
Disease/Mutation information Target Mutation: c.281G>C,p.Gly94Ala
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_008689.1:g.12678G>C
Pathogenic status of mutation: Pathogenic
Disease:Amyotrophic Lateral Sclerosis (ALS)
Clinical Resources ClinVar ID:14760
KEGG disease ID:H00058
OMIM ID: 105400
COSMIC: SOD1
DECIPHER: SOD1
GeneTests: SOD1
ASP siRNA details Mutant allele (5'->3'): UCGAUUCCAUGUUCAUGAG
ASP-siRNA (As strand 3'->5'):AGCUAAGGUACAAGUACUC
Percentage efficacy of ASP-siRNA for mutant allele:50
Wild allele (5'->3'): UGGAUUCCAUGUUCAUGAG
ASP-siRNA (As strand 3'->5'):AGCUAAGGUACAAGUACUC
Percentage efficacy of ASP-siRNA for wild allele:42
Relative difference:8
Wild siRNA details Wild allele (5'->3'): UGGAUUCCAUGUUCAUGAG
Wild siRNA (As strand 3'->5'):ACCUAAGGUACAAGUACUC
Percentage efficacy of wild siRNA for wild allele:NA
Wild allele (5'->3'): UGGAUUCCAUGUUCAUGAG
ASP-siRNA (As strand 3'->5'):AGCUAAGGUACAAGUACUC
Percentage efficacy of Wild sirna for mutant allele:NA
Relative difference :NA
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: Transgenic mouse model
Experimental technique used: rotarod test
Transfection method: RNAiMAX
siRNA expression method: Dharmacon
Post-transfection duration: 168 hours
Concentration used:300nM
Reference:15768029
Delivery method:Transfection
Article title:Five siRNAs targeting three SNPs may provide therapy for three-quarters of Huntington's disease patients.
Authors:Pfister EL, Kennington L, Straubhaar J, Wagh S, Liu W, DiFiglia M, Landwehrmeyer B, Vonsattel JP, Zamore PD, Aronin N.
Journal Reference:Curr Biol. 2009 May 12;19(9):774-8. doi: 10.1016/j.cub.2009.03.030. Epub 2009 Apr 9.