aspsirna0858 details

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ASPsiRNA information siRNA Id:aspsirna0858
siRNA Name: ShR-P12
Mismatch information Wild siRNA (As strand 3'->5'):CGGUCGGCACUUGUUACAG
ASP-siRNA (As strand 3'->5'):CGGUCGGUACUUGUUACAG
Mismatch position in siRNA: U12C
Gene Information Gene Name Ataxin-7(ATXN7)
Target Sequence (5'->3'):AGGUUGCCAAAGUGCCAGCCGUGAACAAUGUCCACAUGA
Wild allele (5'->3'):GCCAGCCGUGAACAAUGUC
Mutant allele (5'->3'):GCCAGCCAUGAACAAUGUC
Position of siRNA on target gene: 3130-3148
Respective Gene/Protein Resources GenBank Accession:NM000333.3
Cytogenic location:3p14.1
Chromosomal coordinates:3:63,849,784-63,989,239
UniProt ID:O15265
HUGO ID:10560
Reference SNp(RefSNP): rs193922929
Disease/Mutation information Target Mutation: c.88_90CAG(7_17)
Matation type/variant: Microsatellite
Mutation at gene level: NG_008227.1:g.53130_53132CAG(7_17)
Pathogenic status of mutation: Pathogenic
Disease:Spino Cerebellar Ataxia type-7 (SCA-7)
Clinical Resources ClinVar ID:2953
KEGG disease ID:H00063
OMIM ID: 164500
COSMIC: ATXN7
DECIPHER: ATXN7
GeneTests: ATXN7
ASP siRNA details Mutant allele (5'->3'): GCCAGCCAUGAACAAUGUC
ASP-siRNA (As strand 3'->5'):CGGUCGGUACUUGUUACAG
Percentage efficacy of ASP-siRNA for mutant allele:83
Wild allele (5'->3'): GCCAGCCGUGAACAAUGUC
ASP-siRNA (As strand 3'->5'):CGGUCGGUACUUGUUACAG
Percentage efficacy of ASP-siRNA for wild allele:30
Relative difference:53
Wild siRNA details Wild allele (5'->3'): GCCAGCCGUGAACAAUGUC
Wild siRNA (As strand 3'->5'):CGGUCGGCACUUGUUACAG
Percentage efficacy of wild siRNA for wild allele:49
Wild allele (5'->3'): GCCAGCCGUGAACAAUGUC
ASP-siRNA (As strand 3'->5'):CGGUCGGUACUUGUUACAG
Percentage efficacy of Wild sirna for mutant allele:20
Relative difference :29
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Constructed by PCR
Post-transfection duration: 48 hours
Concentration used:50nM
Reference:19789634
Delivery method:Transfection
Article title:Design of RNAi hairpins for mutation-specific silencing of ataxin-7 and correction of a SCA7 phenotype.
Authors:Scholefield J, Greenberg LJ, Weinberg MS, Arbuthnot PB, Abdelgany A, Wood MJ.
Journal Reference:PLoS One. 2009 Sep 30;4(9):e7232. doi: 10.1371/journal.pone.0007232.