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| ASPsiRNA information |
siRNA Id: | aspsirna0871
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siRNA Name: | 1
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | GCCCUCUGGCGGUTCCGCU
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ASP-siRNA (As strand 3'->5'): | CCCCUCUGGCGGUTCCGCU
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Mismatch position in siRNA: | C19G |
| Gene Information |
Gene Name | Hungtintin (HTT) |
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Target Sequence (5'->3'): | CGGGAGACCGCCATGGCGACCCT
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Wild allele (5'->3'): | CGGGAGACCGCCATGGCGA
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Mutant allele (5'->3'): | GGGGAGACCGCCATGGCGA
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Position of siRNA on target gene: | 305-323
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_002111.7 |
| Cytogenic location: | 4p16.3 |
| Chromosomal coordinates: | 4:3,076,407-3,245,686 |
| UniProt ID: | Q96CV9 |
| HUGO ID: | 4851 |
|
Reference SNp(RefSNP): | rs19392295 |
| Disease/Mutation information |
Target Mutation: | c.52CAG(36_39)
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Matation type/variant: | Microsatellite
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Mutation at gene level: | NG_009378.1:g.5197CAG(36_39)
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Pathogenic status of mutation: | Pathogenic
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Disease: | Huntington disease (HD) |
| Clinical Resources |
ClinVar ID: | 31916 |
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KEGG disease ID: | H00059 |
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OMIM ID: | 143100 |
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COSMIC: | HTT |
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DECIPHER: | HTT |
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GeneTests: | HTT |
| ASP siRNA details |
Mutant allele (5'->3'): | GGGGAGACCGCCATGGCGA
|
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ASP-siRNA (As strand 3'->5'): | CCCCUCUGGCGGUTCCGCU
|
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Percentage efficacy of ASP-siRNA for mutant allele: | NA
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Wild allele (5'->3'): | CGGGAGACCGCCATGGCGA
|
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ASP-siRNA (As strand 3'->5'): | CCCCUCUGGCGGUTCCGCU
|
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Percentage efficacy of ASP-siRNA for wild allele: | 20
|
|
Relative difference: | NA
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| Wild siRNA details |
Wild allele (5'->3'): | CGGGAGACCGCCATGGCGA
|
|
Wild siRNA (As strand 3'->5'): | GCCCUCUGGCGGUTCCGCU
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | CGGGAGACCGCCATGGCGA
|
|
ASP-siRNA (As strand 3'->5'): | CCCCUCUGGCGGUTCCGCU
|
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
|
Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA
|
| Cell-line used: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Constructed by PCR |
| Post-transfection duration: | 48 hours |
| Concentration used: | 100nM |
| Reference: | 18931668 |
| Delivery method: | Transfection |
| Article title: | Linking SNPs to CAG repeat length in Huntington's disease patients |
| Authors: | Liu W1, Kennington LA, Rosas HD, Hersch S, Cha JH, Zamore PD, Aronin N |
| Journal Reference: | Nat Methods. 2008 Nov;5(11):951-3. doi: 10.1038/nmeth.1261. Epub 2008 Oct 19 |
