| | |
ASPsiRNA information |
siRNA Id: | aspsirna0871
|
siRNA Name: | 1
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | GCCCUCUGGCGGUTCCGCU
|
ASP-siRNA (As strand 3'->5'): | CCCCUCUGGCGGUTCCGCU
|
Mismatch position in siRNA: | C19G |
Gene Information |
Gene Name | Hungtintin (HTT) |
Target Sequence (5'->3'): | CGGGAGACCGCCATGGCGACCCT
|
Wild allele (5'->3'): | CGGGAGACCGCCATGGCGA
|
Mutant allele (5'->3'): | GGGGAGACCGCCATGGCGA
|
Position of siRNA on target gene: | 305-323
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_002111.7 |
Cytogenic location: | 4p16.3 |
Chromosomal coordinates: | 4:3,076,407-3,245,686 |
UniProt ID: | Q96CV9 |
HUGO ID: | 4851 |
Reference SNp(RefSNP): | rs19392295 |
Disease/Mutation information |
Target Mutation: | c.52CAG(36_39)
|
Matation type/variant: | Microsatellite
|
Mutation at gene level: | NG_009378.1:g.5197CAG(36_39)
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Huntington disease (HD) |
Clinical Resources |
ClinVar ID: | 31916 |
KEGG disease ID: | H00059 |
OMIM ID: | 143100 |
COSMIC: | HTT |
DECIPHER: | HTT |
GeneTests: | HTT |
ASP siRNA details |
Mutant allele (5'->3'): | GGGGAGACCGCCATGGCGA
|
ASP-siRNA (As strand 3'->5'): | CCCCUCUGGCGGUTCCGCU
|
Percentage efficacy of ASP-siRNA for mutant allele: | NA
|
Wild allele (5'->3'): | CGGGAGACCGCCATGGCGA
|
ASP-siRNA (As strand 3'->5'): | CCCCUCUGGCGGUTCCGCU
|
Percentage efficacy of ASP-siRNA for wild allele: | 20
|
Relative difference: | NA
|
Wild siRNA details |
Wild allele (5'->3'): | CGGGAGACCGCCATGGCGA
|
Wild siRNA (As strand 3'->5'): | GCCCUCUGGCGGUTCCGCU
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
Wild allele (5'->3'): | CGGGAGACCGCCATGGCGA
|
ASP-siRNA (As strand 3'->5'): | CCCCUCUGGCGGUTCCGCU
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
Relative difference : | NA
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | HEK293 |
Experimental technique used: | Dual luciferase reporter assay |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Constructed by PCR |
Post-transfection duration: | 48 hours |
Concentration used: | 100nM |
Reference: | 18931668 |
Delivery method: | Transfection |
'Article title: | Linking SNPs to CAG repeat length in Huntington's disease patients |
'Authors: | Liu W1, Kennington LA, Rosas HD, Hersch S, Cha JH, Zamore PD, Aronin N |
'Journal Reference: | Nat Methods. 2008 Nov;5(11):951-3. doi: 10.1038/nmeth.1261. Epub 2008 Oct 19 |
'