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| ASPsiRNA information |
siRNA Id: | aspsirna0872
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siRNA Name: | siP1
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | GAGGGCCGUCCACCACCUCG
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ASP-siRNA (As strand 3'->5'): | CAGGGCCGUCCACCACCUCG
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Mismatch position in siRNA: | C19G |
| Gene Information |
Gene Name | Presenilin1 (PSEN1) |
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Target Sequence (5'->3'): | GGTAACTCCCGGCAGGTGGTGGAGCA
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Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
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Mutant allele (5'->3'): | GUCCCGGCAGGUGGUGGAGC
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Position of siRNA on target gene: | 458-477
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000021.3 |
| Cytogenic location: | 14q24.2 |
| Chromosomal coordinates: | 14:73,603,142-73,690,398 |
| UniProt ID: | P49768 |
| HUGO ID: | 9508 |
|
Reference SNp(RefSNP): | 63751441 |
| Disease/Mutation information |
Target Mutation: | c.458C>G, p.Leu153Val
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_007386.2:g.42214C>G
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Pathogenic status of mutation: | Not provided
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Disease: | Alzheimers disease |
| Clinical Resources |
ClinVar ID: | 98031 |
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KEGG disease ID: | H00056 |
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OMIM ID: | 104300 |
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COSMIC: | PSEN1 |
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DECIPHER: | PSEN1 |
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GeneTests: | PSEN1 |
| ASP siRNA details |
Mutant allele (5'->3'): | GUCCCGGCAGGUGGUGGAGC
|
|
ASP-siRNA (As strand 3'->5'): | CAGGGCCGUCCACCACCUCG
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 60
|
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Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
|
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ASP-siRNA (As strand 3'->5'): | CAGGGCCGUCCACCACCUCG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 15
|
|
Relative difference: | 45
|
| Wild siRNA details |
Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
|
|
Wild siRNA (As strand 3'->5'): | GAGGGCCGUCCACCACCUCG
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
|
|
ASP-siRNA (As strand 3'->5'): | CAGGGCCGUCCACCACCUCG
|
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
|
Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA
|
| Cell-line used: | HeLa |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine |
| siRNA expression method: | Constructed by PCR |
| Post-transfection duration: | NA |
| Concentration used: | 100microM |
| Reference: | 18776243 |
| Delivery method: | Transfection |
| Article title: | RNA interference in silencing of genes of Alzheimer's disease in cellular and rat brain models |
| Authors: | Sierant M, Kubiak K, Kazmierczak-Baranska J, Paduszynska A, Kuwabara T, Warashina M, Nacmias B, Sorbi S, Nawrot B |
| Journal Reference: | Nucleic Acids Symp Ser (Oxf). 2008;(52):41-2. doi: 10.1093/nass/nrn021 |
