| | |
|---|
| ASPsiRNA information |
siRNA Id: | aspsirna0874
|
|
siRNA Name: | siP1
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | GAGGGCCGUCCACCACCUCG
|
|
ASP-siRNA (As strand 3'->5'): | CAGGGCCGUCCACCACCUCG
|
|
Mismatch position in siRNA: | C19G |
| Gene Information |
Gene Name | Presenilin1 (PSEN1) |
|
Target Sequence (5'->3'): | GGTAACTCCCGGCAGGTGGTGGAGCA
|
|
Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
|
|
Mutant allele (5'->3'): | GUCCCGGCAGGUGGUGGAGC
|
|
Position of siRNA on target gene: | 458-477
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000021.3 |
| Cytogenic location: | 14q24.2 |
| Chromosomal coordinates: | 14:73,603,142-73,690,398 |
| UniProt ID: | P49768 |
| HUGO ID: | 9508 |
|
Reference SNp(RefSNP): | 63751441 |
| Disease/Mutation information |
Target Mutation: | c.458C>G, p.Leu153Val
|
|
Matation type/variant: | Single nucleotide variant/Missense variant
|
|
Mutation at gene level: | NG_007386.2:g.42214C>G
|
|
Pathogenic status of mutation: | Not provided
|
|
Disease: | Alzheimers disease |
| Clinical Resources |
ClinVar ID: | 98031 |
|
KEGG disease ID: | H00056 |
|
OMIM ID: | 104300 |
|
COSMIC: | PSEN1 |
|
DECIPHER: | PSEN1 |
|
GeneTests: | PSEN1 |
| ASP siRNA details |
Mutant allele (5'->3'): | GUCCCGGCAGGUGGUGGAGC
|
|
ASP-siRNA (As strand 3'->5'): | CAGGGCCGUCCACCACCUCG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 70
|
|
Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
|
|
ASP-siRNA (As strand 3'->5'): | CAGGGCCGUCCACCACCUCG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | 10
|
|
Relative difference: | 60
|
| Wild siRNA details |
Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
|
|
Wild siRNA (As strand 3'->5'): | GAGGGCCGUCCACCACCUCG
|
|
Percentage efficacy of wild siRNA for wild allele: | NA
|
|
Wild allele (5'->3'): | CUCCCGGCAGGUGGUGGAGC
|
|
ASP-siRNA (As strand 3'->5'): | CAGGGCCGUCCACCACCUCG
|
|
Percentage efficacy of Wild sirna for mutant allele: | NA
|
|
Relative difference : | NA
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | Fibroblast |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine |
| siRNA expression method: | Constructed by PCR |
| Post-transfection duration: | NA |
| Concentration used: | 100microM |
| Reference: | 18776243 |
| Delivery method: | Transfection |
'| Article title: | RNA interference in silencing of genes of Alzheimer's disease in cellular and rat brain models |
'| Authors: | Sierant M, Kubiak K, Kazmierczak-Baranska J, Paduszynska A, Kuwabara T, Warashina M, Nacmias B, Sorbi S, Nawrot B |
'| Journal Reference: | Nucleic Acids Symp Ser (Oxf). 2008;(52):41-2. doi: 10.1093/nass/nrn021 |
'
