aspsirna0883 details

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ASPsiRNA information siRNA Id:aspsirna0883
siRNA Name: K6a_513a.8
Mismatch information Wild siRNA (As strand 3'->5'):UUCUGGGAGUUGUUGUUCA
ASP-siRNA (As strand 3'->5'):UUCUGGGAGUUUUUGUUCA
Mismatch position in siRNA: U8C
Gene Information Gene Name Keratin 6a (KRT6a)
Target Sequence (5'->3'):AACAGATCAAGACCCTCAACAACAAGTTTGCCTC
Wild allele (5'->3'):AAGACCCUCAACAACAAGU
Mutant allele (5'->3'):AAGACCCUCAAAAACAAGU
Position of siRNA on target gene: 721-739
Respective Gene/Protein Resources GenBank Accession:NM_005554.3
Cytogenic location:12q13.13
Chromosomal coordinates:12:52,880,957-52,887,180
UniProt ID:P02538
HUGO ID:6443
Reference SNp(RefSNP): rs59685571
Disease/Mutation information Target Mutation: c.513C>A,p.Asn171Lys
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_008298.1:g.5722C>A
Pathogenic status of mutation: Disease causing N171K mutation in K6a gene
Disease:Pachyonychia congenita (PC)
Clinical Resources ClinVar ID:66589
KEGG disease ID:H00684
OMIM ID: 167200
COSMIC: KRT6a
DECIPHER: KRT6a
GeneTests: KRT6a
ASP siRNA details Mutant allele (5'->3'): AAGACCCUCAAAAACAAGU
ASP-siRNA (As strand 3'->5'):UUCUGGGAGUUUUUGUUCA
Percentage efficacy of ASP-siRNA for mutant allele:33
Wild allele (5'->3'): AAGACCCUCAACAACAAGU
ASP-siRNA (As strand 3'->5'):UUCUGGGAGUUUUUGUUCA
Percentage efficacy of ASP-siRNA for wild allele:0
Relative difference:33
Wild siRNA details Wild allele (5'->3'): AAGACCCUCAACAACAAGU
Wild siRNA (As strand 3'->5'):UUCUGGGAGUUGUUGUUCA
Percentage efficacy of wild siRNA for wild allele:46
Wild allele (5'->3'): AAGACCCUCAACAACAAGU
ASP-siRNA (As strand 3'->5'):UUCUGGGAGUUUUUGUUCA
Percentage efficacy of Wild sirna for mutant allele:12
Relative difference :34
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: 293T
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine 2000
siRNA expression method: Dharmacon
Post-transfection duration: 48 hours
Concentration used:150ng
Reference:17914454
Delivery method:Transfection
Article title:Single-nucleotide-specific siRNA targeting in a dominant-negative skin model.
Authors:Hickerson RP, Smith FJ, Reeves RE, Contag CH, Leake D, Leachman SA, Milstone LM, McLean WH, Kaspar RL.
Journal Reference:J Invest Dermatol. 2008 Mar;128(3):594-605. Epub 2007 Oct 11.