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| ASPsiRNA information |
siRNA Id: | aspsirna0902
|
|
siRNA Name: | siAhc04
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | UCUAUGUGGACGGUACACG
|
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ASP-siRNA (As strand 3'->5'): | UCUAUGAGGACGGUACACG
|
|
Mismatch position in siRNA: | A13U |
| Gene Information |
Gene Name | MHC, class I, A (HLA-A) heavy chain |
|
Target Sequence (5'->3'): | CGGTTCGACAGCGACGCCGCG
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Wild allele (5'->3'): | AGAUACACCUGCCAUGUGC
|
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Mutant allele (5'->3'): | AGAUACUCCUGCCAUGUGC
|
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Position of siRNA on target gene: | 922-940
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_001242758.1 |
| Cytogenic location: | 6p22.1 |
| Chromosomal coordinates: | 6:29,910,246-29,913,660 |
| UniProt ID: | P01892 |
| HUGO ID: | 4931 |
|
Reference SNp(RefSNP): | rs1061235 |
| Disease/Mutation information |
Target Mutation: | c.*66A>T
|
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Matation type/variant: | Single nucleotide variant/3 prime UTR variant
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Mutation at gene level: | NG_029217.2:g.8057A>T
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Pathogenic status of mutation: | Risk factor
|
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Disease: | HLA incompatibility |
| Clinical Resources |
ClinVar ID: | 29755 |
|
KEGG disease ID: | NA |
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OMIM ID: | NA |
|
COSMIC: | HLA-A heavy chain |
|
DECIPHER: | HLA-A heavy chain |
|
GeneTests: | HLA-A heavy chain |
| ASP siRNA details |
Mutant allele (5'->3'): | AGAUACUCCUGCCAUGUGC
|
|
ASP-siRNA (As strand 3'->5'): | UCUAUGAGGACGGUACACG
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 70
|
|
Wild allele (5'->3'): | AGAUACACCUGCCAUGUGC
|
|
ASP-siRNA (As strand 3'->5'): | UCUAUGAGGACGGUACACG
|
|
Percentage efficacy of ASP-siRNA for wild allele: | NA
|
|
Relative difference: | NA
|
| Wild siRNA details |
Wild allele (5'->3'): | AGAUACACCUGCCAUGUGC
|
|
Wild siRNA (As strand 3'->5'): | UCUAUGUGGACGGUACACG
|
|
Percentage efficacy of wild siRNA for wild allele: | 50
|
|
Wild allele (5'->3'): | AGAUACACCUGCCAUGUGC
|
|
ASP-siRNA (As strand 3'->5'): | UCUAUGAGGACGGUACACG
|
|
Percentage efficacy of Wild sirna for mutant allele: | 90
|
|
Relative difference : | -40
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | Epstein–Barr-virus-immortalized B-lymphocytes |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiFect |
| siRNA expression method: | Ambion |
| Post-transfection duration: | 48 hours |
| Concentration used: | 1.2mIcrog |
| Reference: | 16520945 |
| Delivery method: | Lentiviral vector delivery |
'| Article title: | Class-, gene-, and group-specific HLA silencing by lentiviral shRNA delivery. |
'| Authors: | Figueiredo C, Seltsam A, Blasczyk R. |
'| Journal Reference: | J Mol Med (Berl). 2006 May;84(5):425-37. Epub 2006 Mar 7. |
'
