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| ASPsiRNA information |
siRNA Id: | aspsirna0908
|
|
siRNA Name: | Sibeta2m05
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | CCAAAUGAGUGCAGUAGGU
|
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ASP-siRNA (As strand 3'->5'): | CCAAAUGAGUGGAGUAGGU
|
|
Mismatch position in siRNA: | G8C |
| Gene Information |
Gene Name | Beta 2-microglobulin (B2M) |
|
Target Sequence (5'->3'): | ATTGAAAAAGTGGAGCATTCAGACT
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Wild allele (5'->3'): | GGUUUACUCACGUCAUCCA
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Mutant allele (5'->3'): | GGUUUACUCACCUCAUCCA
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Position of siRNA on target gene: | 144-162
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_004048.2 |
| Cytogenic location: | 15q21.1 |
| Chromosomal coordinates: | 15:45,003,684-45,010,356 |
| UniProt ID: | P61769 |
| HUGO ID: | 914 |
|
Reference SNp(RefSNP): | rs10489448 |
| Disease/Mutation information |
Target Mutation: | c.31G>C,p.Ala11Pro
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_012920.1:g.5091G>C
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Pathogenic status of mutation: | Pathogenic
|
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Disease: | HLA incompatibility |
| Clinical Resources |
ClinVar ID: | 17740 |
|
KEGG disease ID: | NA |
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OMIM ID: | NA |
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COSMIC: | B2M |
|
DECIPHER: | B2M |
|
GeneTests: | B2M |
| ASP siRNA details |
Mutant allele (5'->3'): | GGUUUACUCACCUCAUCCA
|
|
ASP-siRNA (As strand 3'->5'): | CCAAAUGAGUGGAGUAGGU
|
|
Percentage efficacy of ASP-siRNA for mutant allele: | 70
|
|
Wild allele (5'->3'): | GGUUUACUCACGUCAUCCA
|
|
ASP-siRNA (As strand 3'->5'): | CCAAAUGAGUGGAGUAGGU
|
|
Percentage efficacy of ASP-siRNA for wild allele: | NA
|
|
Relative difference: | NA
|
| Wild siRNA details |
Wild allele (5'->3'): | GGUUUACUCACGUCAUCCA
|
|
Wild siRNA (As strand 3'->5'): | CCAAAUGAGUGCAGUAGGU
|
|
Percentage efficacy of wild siRNA for wild allele: | 50
|
|
Wild allele (5'->3'): | GGUUUACUCACGUCAUCCA
|
|
ASP-siRNA (As strand 3'->5'): | CCAAAUGAGUGGAGUAGGU
|
|
Percentage efficacy of Wild sirna for mutant allele: | 90
|
|
Relative difference : | -40
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | Epstein–Barr-virus-immortalized B-lymphocytes |
| Experimental technique used: | Western blot |
| Transfection method: | RNAiFect |
| siRNA expression method: | Ambion |
| Post-transfection duration: | 48 hours |
| Concentration used: | 1.2mIcrog |
| Reference: | 16520945 |
| Delivery method: | Lentiviral vector delivery |
'| Article title: | Class-, gene-, and group-specific HLA silencing by lentiviral shRNA delivery. |
'| Authors: | Figueiredo C, Seltsam A, Blasczyk R. |
'| Journal Reference: | J Mol Med (Berl). 2006 May;84(5):425-37. Epub 2006 Mar 7. |
'
