aspsirna0914 details

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ASPsiRNA information siRNA Id:aspsirna0914
siRNA Name: siAhc04
Mismatch information Wild siRNA (As strand 3'->5'):UCUAUGUGGACGGUACACG
ASP-siRNA (As strand 3'->5'):UCUAUGAGGACGGUACACG
Mismatch position in siRNA: A13U
Gene Information Gene Name MHC, class I, A (HLA-A) heavy chain
Target Sequence (5'->3'):CGGTTCGACAGCGACGCCGCG
Wild allele (5'->3'):AGAUACACCUGCCAUGUGC
Mutant allele (5'->3'):AGAUACUCCUGCCAUGUGC
Position of siRNA on target gene: 922-940
Respective Gene/Protein Resources GenBank Accession:NM_001242758.1
Cytogenic location:6p22.1
Chromosomal coordinates:6:29,910,246-29,913,660
UniProt ID:P01892
HUGO ID:4931
Reference SNp(RefSNP): rs1061235
Disease/Mutation information Target Mutation: c.*66A>T
Matation type/variant: Single nucleotide variant/3 prime UTR variant
Mutation at gene level: NG_029217.2:g.8057A>T
Pathogenic status of mutation: Risk factor
Disease:HLA incompatibility
Clinical Resources ClinVar ID:29755
KEGG disease ID:NA
OMIM ID: NA
COSMIC: HLA-A heavy chain
DECIPHER: HLA-A heavy chain
GeneTests: HLA-A heavy chain
ASP siRNA details Mutant allele (5'->3'): AGAUACUCCUGCCAUGUGC
ASP-siRNA (As strand 3'->5'):UCUAUGAGGACGGUACACG
Percentage efficacy of ASP-siRNA for mutant allele:75
Wild allele (5'->3'): AGAUACACCUGCCAUGUGC
ASP-siRNA (As strand 3'->5'):UCUAUGAGGACGGUACACG
Percentage efficacy of ASP-siRNA for wild allele:NA
Relative difference:NA
Wild siRNA details Wild allele (5'->3'): AGAUACACCUGCCAUGUGC
Wild siRNA (As strand 3'->5'):UCUAUGUGGACGGUACACG
Percentage efficacy of wild siRNA for wild allele:50
Wild allele (5'->3'): AGAUACACCUGCCAUGUGC
ASP-siRNA (As strand 3'->5'):UCUAUGAGGACGGUACACG
Percentage efficacy of Wild sirna for mutant allele:90
Relative difference :-40
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Western blot
Transfection method: RNAiFect
siRNA expression method: Ambion
Post-transfection duration: 48 hours
Concentration used:1.2mIcrog
Reference:16520945
Delivery method:Lentiviral vector delivery
Article title:Class-, gene-, and group-specific HLA silencing by lentiviral shRNA delivery.
Authors:Figueiredo C, Seltsam A, Blasczyk R.
Journal Reference:J Mol Med (Berl). 2006 May;84(5):425-37. Epub 2006 Mar 7.