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| ASPsiRNA information |
siRNA Id: | aspsirna0932
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siRNA Name: | HWT-G93A-266
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | GACGACUGUUUCUACCACA
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ASP-siRNA (As strand 3'->5'): | GACGACUGUUUCUACGACA
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Mismatch position in siRNA: | G4C |
| Gene Information |
Gene Name | Cu,Zn superoxide dismutase (SOD1) |
|
Target Sequence (5'->3'): | GACTTGGGCAATGTGACTGCTGACAAAGAUGGUGUGGCCGAU
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Wild allele (5'->3'): | CUGCUGACAAAGAUGGUGU
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Mutant allele (5'->3'): | CUGCUGACAAAGAUGCUGU
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Position of siRNA on target gene: | 414-432
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_000454.4 |
| Cytogenic location: | 21q22.11 |
| Chromosomal coordinates: | 21:33,031,934-33,041,243 |
| UniProt ID: | P00441 |
| HUGO ID: | 11179 |
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Reference SNp(RefSNP): | rs12191243 |
| Disease/Mutation information |
Target Mutation: | c.281G>C (p.Gly94Ala)
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_008689.1:g.12678G>C
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Pathogenic status of mutation: | Pathogenic
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Disease: | Amyotrophic Lateral Sclerosis (ALS) |
| Clinical Resources |
ClinVar ID: | 14760 |
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KEGG disease ID: | H00058 |
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OMIM ID: | 105400 |
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COSMIC: | SOD1 |
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DECIPHER: | SOD1 |
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GeneTests: | SOD1 |
| ASP siRNA details |
Mutant allele (5'->3'): | CUGCUGACAAAGAUGCUGU
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ASP-siRNA (As strand 3'->5'): | GACGACUGUUUCUACGACA
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 85
|
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Wild allele (5'->3'): | CUGCUGACAAAGAUGGUGU
|
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ASP-siRNA (As strand 3'->5'): | GACGACUGUUUCUACGACA
|
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Percentage efficacy of ASP-siRNA for wild allele: | 90
|
|
Relative difference: | -5
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| Wild siRNA details |
Wild allele (5'->3'): | CUGCUGACAAAGAUGGUGU
|
|
Wild siRNA (As strand 3'->5'): | GACGACUGUUUCUACCACA
|
|
Percentage efficacy of wild siRNA for wild allele: | 30
|
|
Wild allele (5'->3'): | CUGCUGACAAAGAUGGUGU
|
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ASP-siRNA (As strand 3'->5'): | GACGACUGUUUCUACGACA
|
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Percentage efficacy of Wild sirna for mutant allele: | 85
|
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Relative difference : | -55
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA
|
| Cell-line used: | HeLa |
| Experimental technique used: | Flourescence microscopy |
| Transfection method: | Lipofectamine 2000 |
| siRNA expression method: | Qiagen |
| Post-transfection duration: | 24/48/72 hours |
| Concentration used: | 0.03microg |
| Reference: | 14981234 |
| Delivery method: | Transfection |
| Article title: | RNA interference-mediated silencing of mutant superoxide dismutase rescues cyclosporin A-induced death in cultured neuroblastoma cells. |
| Authors: | Maxwell MM, Pasinelli P, Kazantsev AG, Brown RH Jr |
| Journal Reference: | Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3178-83. Epub 2004 Feb 23 |
