aspsirna0933 details

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ASPsiRNA information siRNA Id:aspsirna0933
siRNA Name: HWT-G93A-273
Mismatch information Wild siRNA (As strand 3'->5'):GUUUCUACCACACCGGCUA
ASP-siRNA (As strand 3'->5'):GUUUCUACGACACCGGCUA
Mismatch position in siRNA: G11C
Gene Information Gene Name Cu,Zn superoxide dismutase (SOD1)
Target Sequence (5'->3'):GACTTGGGCAATGTGACTGCTGACAAAGAUGGUGUGGCCGAU
Wild allele (5'->3'):CAAAGAUGGUGUGGCCGAU
Mutant allele (5'->3'):CAAAGAUGCUGUGGCCGAU
Position of siRNA on target gene: 421-439
Respective Gene/Protein Resources GenBank Accession:NM_000454.4
Cytogenic location:21q22.11
Chromosomal coordinates:21:33,031,934-33,041,243
UniProt ID:P00441
HUGO ID:11179
Reference SNp(RefSNP): rs12191243
Disease/Mutation information Target Mutation: c.281G>C (p.Gly94Ala)
Matation type/variant: Single nucleotide variant/Missense variant
Mutation at gene level: NG_008689.1:g.12678G>C
Pathogenic status of mutation: Pathogenic
Disease:Amyotrophic Lateral Sclerosis (ALS)
Clinical Resources ClinVar ID:14760
KEGG disease ID:H00058
OMIM ID: 105400
COSMIC: SOD1
DECIPHER: SOD1
GeneTests: SOD1
ASP siRNA details Mutant allele (5'->3'): CAAAGAUGCUGUGGCCGAU
ASP-siRNA (As strand 3'->5'):GUUUCUACGACACCGGCUA
Percentage efficacy of ASP-siRNA for mutant allele:78
Wild allele (5'->3'): CAAAGAUGGUGUGGCCGAU
ASP-siRNA (As strand 3'->5'):GUUUCUACGACACCGGCUA
Percentage efficacy of ASP-siRNA for wild allele:65
Relative difference:13
Wild siRNA details Wild allele (5'->3'): CAAAGAUGGUGUGGCCGAU
Wild siRNA (As strand 3'->5'):GUUUCUACCACACCGGCUA
Percentage efficacy of wild siRNA for wild allele:30
Wild allele (5'->3'): CAAAGAUGGUGUGGCCGAU
ASP-siRNA (As strand 3'->5'):GUUUCUACGACACCGGCUA
Percentage efficacy of Wild sirna for mutant allele:85
Relative difference :-55
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HeLa
Experimental technique used: Flourescence microscopy
Transfection method: Lipofectamine 2000
siRNA expression method: Qiagen
Post-transfection duration: 24/48/72 hours
Concentration used:0.03microg
Reference:14981234
Delivery method:Transfection
Article title:RNA interference-mediated silencing of mutant superoxide dismutase rescues cyclosporin A-induced death in cultured neuroblastoma cells.
Authors:Maxwell MM, Pasinelli P, Kazantsev AG, Brown RH Jr
Journal Reference:Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3178-83. Epub 2004 Feb 23