| | |
ASPsiRNA information |
siRNA Id: | aspsirna0934
|
siRNA Name: | HWT-G93A-273
|
Mismatch information |
Wild siRNA (As strand 3'->5'): | GUUUCUACCACACCGGCUA
|
ASP-siRNA (As strand 3'->5'): | GUUUCUACGACACCGGCUA
|
Mismatch position in siRNA: | G11C |
Gene Information |
Gene Name | Cu,Zn superoxide dismutase (SOD1) |
Target Sequence (5'->3'): | GACTTGGGCAATGTGACTGCTGACAAAGAUGGUGUGGCCGAU
|
Wild allele (5'->3'): | CAAAGAUGGUGUGGCCGAU
|
Mutant allele (5'->3'): | CAAAGAUGCUGUGGCCGAU
|
Position of siRNA on target gene: | 421-439
|
Respective Gene/Protein Resources |
GenBank Accession: | NM_000454.4 |
Cytogenic location: | 21q22.11 |
Chromosomal coordinates: | 21:33,031,934-33,041,243 |
UniProt ID: | P00441 |
HUGO ID: | 11179 |
Reference SNp(RefSNP): | rs12191243 |
Disease/Mutation information |
Target Mutation: | c.281G>C (p.Gly94Ala)
|
Matation type/variant: | Single nucleotide variant/Missense variant
|
Mutation at gene level: | NG_008689.1:g.12678G>C
|
Pathogenic status of mutation: | Pathogenic
|
Disease: | Amyotrophic Lateral Sclerosis (ALS) |
Clinical Resources |
ClinVar ID: | 14760 |
KEGG disease ID: | H00058 |
OMIM ID: | 105400 |
COSMIC: | SOD1 |
DECIPHER: | SOD1 |
GeneTests: | SOD1 |
ASP siRNA details |
Mutant allele (5'->3'): | CAAAGAUGCUGUGGCCGAU
|
ASP-siRNA (As strand 3'->5'): | GUUUCUACGACACCGGCUA
|
Percentage efficacy of ASP-siRNA for mutant allele: | 80
|
Wild allele (5'->3'): | CAAAGAUGGUGUGGCCGAU
|
ASP-siRNA (As strand 3'->5'): | GUUUCUACGACACCGGCUA
|
Percentage efficacy of ASP-siRNA for wild allele: | 34
|
Relative difference: | 46
|
Wild siRNA details |
Wild allele (5'->3'): | CAAAGAUGGUGUGGCCGAU
|
Wild siRNA (As strand 3'->5'): | GUUUCUACCACACCGGCUA
|
Percentage efficacy of wild siRNA for wild allele: | 30
|
Wild allele (5'->3'): | CAAAGAUGGUGUGGCCGAU
|
ASP-siRNA (As strand 3'->5'): | GUUUCUACGACACCGGCUA
|
Percentage efficacy of Wild sirna for mutant allele: | 85
|
Relative difference : | -55
|
General information |
Mismatch incorporated in siRNA/Target: | siRNA |
Cell-line used: | HeLa |
Experimental technique used: | Flourescence microscopy |
Transfection method: | Lipofectamine 2000 |
siRNA expression method: | Qiagen |
Post-transfection duration: | 24/48/72 hours |
Concentration used: | 0.03microg |
Reference: | 14981234 |
Delivery method: | Transfection |
'Article title: | RNA interference-mediated silencing of mutant superoxide dismutase rescues cyclosporin A-induced death in cultured neuroblastoma cells. |
'Authors: | Maxwell MM, Pasinelli P, Kazantsev AG, Brown RH Jr |
'Journal Reference: | Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3178-83. Epub 2004 Feb 23 |
'