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| ASPsiRNA information |
siRNA Id: | aspsirna0935
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siRNA Name: | P-SUPER K-RAS V12
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| Mismatch information |
Wild siRNA (As strand 3'->5'): | CAACCUCGACCACCGCAUC
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ASP-siRNA (As strand 3'->5'): | CAACCUCGACAACCGCAUC
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Mismatch position in siRNA: | A9C |
| Gene Information |
Gene Name | K-RAS (KRAS) |
|
Target Sequence (5'->3'): | GTTGGAGCTGTTGGCGTAG
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Wild allele (5'->3'): | GUUGGAGCUGGUGGCGUAG
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Mutant allele (5'->3'): | GUUGGAGCUGUUGGCGUAG
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Position of siRNA on target gene: | 206-224
|
| Respective Gene/Protein Resources |
GenBank Accession: | NM_033360.2 |
| Cytogenic location: | 12p12.1 |
| Chromosomal coordinates: | 12:25,358,179-25,403,869 |
| UniProt ID: | P01116 |
| HUGO ID: | 6407 |
|
Reference SNp(RefSNP): | rs12191352 |
| Disease/Mutation information |
Target Mutation: | c.35G>T,p.Gly12Val
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Matation type/variant: | Single nucleotide variant/Missense variant
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Mutation at gene level: | NG_007524.1:g.10571G>T
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Pathogenic status of mutation: | Pathogenic
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Disease: | Cancer |
| Clinical Resources |
ClinVar ID: | 12583 |
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KEGG disease ID: | H00013 |
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OMIM ID: | 211980 |
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COSMIC: | KRAS |
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DECIPHER: | KRAS |
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GeneTests: | KRAS |
| ASP siRNA details |
Mutant allele (5'->3'): | GUUGGAGCUGUUGGCGUAG
|
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ASP-siRNA (As strand 3'->5'): | CAACCUCGACAACCGCAUC
|
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Percentage efficacy of ASP-siRNA for mutant allele: | NA
|
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Wild allele (5'->3'): | GUUGGAGCUGGUGGCGUAG
|
|
ASP-siRNA (As strand 3'->5'): | CAACCUCGACAACCGCAUC
|
|
Percentage efficacy of ASP-siRNA for wild allele: | NA
|
|
Relative difference: | NA
|
| Wild siRNA details |
Wild allele (5'->3'): | GUUGGAGCUGGUGGCGUAG
|
|
Wild siRNA (As strand 3'->5'): | CAACCUCGACCACCGCAUC
|
|
Percentage efficacy of wild siRNA for wild allele: | 50
|
|
Wild allele (5'->3'): | GUUGGAGCUGGUGGCGUAG
|
|
ASP-siRNA (As strand 3'->5'): | CAACCUCGACAACCGCAUC
|
|
Percentage efficacy of Wild sirna for mutant allele: | 29
|
|
Relative difference : | 21
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA
|
| Cell-line used: | CAPAN-1 |
| Experimental technique used: | Western blot |
| Transfection method: | Polybrene |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 48 hours |
| Concentration used: | 0.03microg |
| Reference: | 12242156 |
| Delivery method: | Transfection |
| Article title: | Stable suppression of tumorigenicity by virus-mediated RNA interference |
| Authors: | Brummelkamp TR, Bernards R, Agami R |
| Journal Reference: | Cancer Cell. 2002 Sep;2(3):243-7 |
