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| ASPsiRNA information |
siRNA Id: | aspsirna0939
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siRNA Name: | Cat 78 nt
|
| Mismatch information |
Wild siRNA (As strand 3'->5'): | UACAGGAAGGUCGUCGUCG
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ASP-siRNA (As strand 3'->5'): | UACAGGAAGGCCGUCGUCG
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Mismatch position in siRNA: | C9U |
| Gene Information |
Gene Name | Androgen receptor (AR) |
|
Target Sequence (5'->3'): | CAUCGCUCUGGAGUGAAUACC
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Wild allele (5'->3'): | AUGUCCUUCCAGCAGCAGC
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Mutant allele (5'->3'): | AUGUCCUUCCGGCAGCAGC
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Position of siRNA on target gene: | NA
|
| Respective Gene/Protein Resources |
GenBank Accession: | NA |
| Cytogenic location: | Xq12 |
| Chromosomal coordinates: | X:66,763,873-66,950,460 |
| UniProt ID: | P10275 |
| HUGO ID: | 644 |
|
Reference SNp(RefSNP): | rs19392293 |
| Disease/Mutation information |
Target Mutation: | c.172_174CAG(10_36) (p.Gln69_Gln80del)]
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Matation type/variant: | Microsatellite
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Mutation at gene level: | NC_000023.11:g.67545318_67545320(38_?) (GRCh38)
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Pathogenic status of mutation: | Pathogenic
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Disease: | Spinal and Bulbar Muscular Atrophy, X-linked 1; SMAX1/Kennedy’s disease |
| Clinical Resources |
ClinVar ID: | RCV000010492.1 |
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KEGG disease ID: | H00062 |
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OMIM ID: | 313200 |
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COSMIC: | AR |
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DECIPHER: | AR |
|
GeneTests: | AR |
| ASP siRNA details |
Mutant allele (5'->3'): | AUGUCCUUCCGGCAGCAGC
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ASP-siRNA (As strand 3'->5'): | UACAGGAAGGCCGUCGUCG
|
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Percentage efficacy of ASP-siRNA for mutant allele: | 70
|
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Wild allele (5'->3'): | AUGUCCUUCCAGCAGCAGC
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ASP-siRNA (As strand 3'->5'): | UACAGGAAGGCCGUCGUCG
|
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Percentage efficacy of ASP-siRNA for wild allele: | 45
|
|
Relative difference: | 25
|
| Wild siRNA details |
Wild allele (5'->3'): | AUGUCCUUCCAGCAGCAGC
|
|
Wild siRNA (As strand 3'->5'): | UACAGGAAGGUCGUCGUCG
|
|
Percentage efficacy of wild siRNA for wild allele: | Moderately effective
|
|
Wild allele (5'->3'): | AUGUCCUUCCAGCAGCAGC
|
|
ASP-siRNA (As strand 3'->5'): | UACAGGAAGGCCGUCGUCG
|
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Percentage efficacy of Wild sirna for mutant allele: | Low efficacy
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Relative difference : | Poor discrimination
|
| General information |
Mismatch incorporated in siRNA/Target: | siRNA |
| Cell-line used: | HEK293 |
| Experimental technique used: | Dual luciferase reporter assay |
| Transfection method: | Lipofectamine Plus |
| siRNA expression method: | Chemically synthesized |
| Post-transfection duration: | 72 hours |
| Concentration used: | 1mIcrog |
| Reference: | 11809726 |
| Delivery method: | Transfection |
'| Article title: | Rescue of polyglutamine-mediated cytotoxicity by double-stranded RNA-mediated RNA interference. |
'| Authors: | Caplen NJ, Taylor JP, Statham VS, Tanaka F, Fire A, Morgan RA. |
'| Journal Reference: | Hum Mol Genet. 2002 Jan 15;11(2):175-84. |
'
