aspsirna0942 details

''''
ASPsiRNA information siRNA Id:aspsirna0942
siRNA Name: Cag27 81 nt
Mismatch information Wild siRNA (As strand 3'->5'):UACAGGAAGGACGUCGUCG
ASP-siRNA (As strand 3'->5'):UACCGGAAGGACGUCGUCG
Mismatch position in siRNA: C16A
Gene Information Gene Name Androgen receptor (AR)
Target Sequence (5'->3'):CAGCAGCAGCAGCAGCAGCAG
Wild allele (5'->3'):AUGUCCUUCCUGCAGCAGC
Mutant allele (5'->3'):AUGGCCUUCCUGCAGCAGC
Position of siRNA on target gene: NA
Respective Gene/Protein Resources GenBank Accession:NA
Cytogenic location:Xq12
Chromosomal coordinates:X:66,763,873-66,950,460
UniProt ID:P10275
HUGO ID:644
Reference SNp(RefSNP): rs19392293
Disease/Mutation information Target Mutation: c.172_174CAG(10_36) (p.Gln69_Gln80del)]
Matation type/variant: Microsatellite
Mutation at gene level: NC_000023.11:g.67545318_67545320(38_?) (GRCh38)
Pathogenic status of mutation: Pathogenic
Disease:Spinal and Bulbar Muscular Atrophy, X-linked 1; SMAX1/Kennedy’s disease
Clinical Resources ClinVar ID:RCV000010492.1
KEGG disease ID:H00062
OMIM ID: 313200
COSMIC: AR
DECIPHER: AR
GeneTests: AR
ASP siRNA details Mutant allele (5'->3'): AUGGCCUUCCUGCAGCAGC
ASP-siRNA (As strand 3'->5'):UACCGGAAGGACGUCGUCG
Percentage efficacy of ASP-siRNA for mutant allele:77
Wild allele (5'->3'): AUGUCCUUCCUGCAGCAGC
ASP-siRNA (As strand 3'->5'):UACCGGAAGGACGUCGUCG
Percentage efficacy of ASP-siRNA for wild allele:12
Relative difference:65
Wild siRNA details Wild allele (5'->3'): AUGUCCUUCCUGCAGCAGC
Wild siRNA (As strand 3'->5'):UACAGGAAGGACGUCGUCG
Percentage efficacy of wild siRNA for wild allele:Moderately effective
Wild allele (5'->3'): AUGUCCUUCCUGCAGCAGC
ASP-siRNA (As strand 3'->5'):UACCGGAAGGACGUCGUCG
Percentage efficacy of Wild sirna for mutant allele:Low efficacy
Relative difference :Poor discrimination
General information Mismatch incorporated in siRNA/Target:siRNA
Cell-line used: HEK293
Experimental technique used: Dual luciferase reporter assay
Transfection method: Lipofectamine Plus
siRNA expression method: Chemically synthesized
Post-transfection duration: 72 hours
Concentration used:1mIcrog
Reference:11809726
Delivery method:Transfection
Article title:Rescue of polyglutamine-mediated cytotoxicity by double-stranded RNA-mediated RNA interference.
Authors:Caplen NJ, Taylor JP, Statham VS, Tanaka F, Fire A, Morgan RA.
Journal Reference:Hum Mol Genet. 2002 Jan 15;11(2):175-84.